Antifungal activity and mode of action of silver nano-particles on Candida albicans

In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis...

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Bibliographic Details
Published in:Biometals 2009-04, Vol.22 (2), p.235-242
Main Authors: Kim, Keuk-Jun, Sung, Woo Sang, Suh, Bo Kyoung, Moon, Seok-Ki, Choi, Jong-Soo, Kim, Jong Guk, Lee, Dong Gun
Format: Article
Language:English
Subjects:
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Biochemistry
Biomedical and Life Sciences
Candida albicans
Candida albicans - drug effects
Candida albicans - metabolism
Cell Biology
Cell Cycle
Cell Membrane - drug effects
Erythrocytes
Erythrocytes - drug effects
Flow Cytometry - methods
Fungi
Glucose - metabolism
Humans
Life Sciences
Medicine/Public Health
Membranes
Metal Nanoparticles - chemistry
Microbiology
Microscopy, Electron, Transmission - methods
Mode of action
Nanoparticles
Nanotechnology - methods
Pharmacology/Toxicology
Plant Physiology
Saccharomyces cerevisiae - metabolism
Silver
Silver - chemistry
Trehalose - metabolism
Trichosporon - metabolism
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Summary:In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-008-9159-2