Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge

• Published in: Innate immunity (London, England) England), 2009-04, Vol.15 (2), p.81-89
• Main Authors: Carroll, Jeffery A., Reuter, Richard R., Chase, Chadwick C., Coleman, Samuel W., Riley, David G., Spiers, Donald E., Arthington, John D., Galyean, Michael L.
• Format: Article
• Language: English
• Subjects: Acute-Phase Reaction - blood; Acute-Phase Reaction - genetics; Acute-Phase Reaction - immunology; Acute-Phase Reaction - physiopathology; Animals; Cattle; Gene Expression Regulation - immunology; Hydrocortisone - blood; Hydrocortisone - genetics; Hydrocortisone - immunology; Inflammation Mediators - immunology; Inflammation Mediators - metabolism; Injections, Intravenous; Interferon-gamma - immunology; Interferon-gamma - secretion; Interleukin-1beta - immunology; Interleukin-1beta - secretion; Interleukin-6 - immunology; Interleukin-6 - secretion; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - immunology; Male; Protein Array Analysis; Serum Amyloid A Protein - genetics; Serum Amyloid A Protein - immunology; Serum Amyloid A Protein - metabolism; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Tumor Necrosis Factor-alpha - secretion
• ISSN: 1753-4259; 1753-4267
• DOI: 10.1177/1753425908099170

Our objective was to characterize further the acute-phase response following endotoxin (i.e. lipopolysaccharide; LPS) exposure in the bovine. Nine pure-bred Angus castrated males (i.e. steers; average body weight = 299 ± 5 kg) were used in a randomized complete block design in environmentally controlled chambers, set at thermoneutral level, to characterize the acute physiological, endocrine, immune, and acute-phase protein responses following an i.v. bolus administration of 2.5 μg of LPS/kg body weight. One day before administration of LPS, all steers were fitted with an indwelling jugular vein catheter for serial blood collection. Blood samples were collected at 30-min intervals from -2 h to 8 h relative to the LPS challenge (time 0), and serum was harvested and stored at -80 °C until analyzed for concentrations of cortisol, pro-inflammatory cytokines, and acute-phase proteins. Indicators of thermal status (i.e. rectal temperature, ruminal temperature, respiration rate, sweat rate, and skin temperatures) were measured at 30-min intervals from -1 h to 6 h relative to the challenge. Endotoxin exposure increased (P