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Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma
Purpose: We previously identified mina53 , a novel Myc target gene. Here we investigated whether mina53 is related to esophageal squamous cell carcinoma (ESCC), a disease with poor prognosis. Experimental Design: Mina53 expression was suppressed in ESCC cell lines by a RNA interference method to inv...
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Published in: | Clinical cancer research 2004-11, Vol.10 (21), p.7347-7356 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Purpose: We previously identified mina53 , a novel Myc target gene. Here we investigated whether mina53 is related to esophageal squamous cell carcinoma (ESCC), a disease with poor prognosis.
Experimental Design: Mina53 expression was suppressed in ESCC cell lines by a RNA interference method to investigate whether Mina53 is involved
in cell proliferation. Expression of Mina53 was investigated by Western blotting in tissue sections from patients with ESCC.
Immunohistochemical analysis of Mina53 was carried out and compared with that using anti–Ki-67 antibody. Finally, the level
of Mina53 expression was compared with the length of survival of patients with ESCC.
Results: Reduction of mina53 expression by RNA interference suppressed cell proliferation in ESCC cell lines. Western blot analysis of surgically resected
ESCC specimens indicated that the expression of Mina53 in tumors was increased compared with that in adjacent nonneoplastic
tissues in all four specimens examined. When formalin-fixed specimens from 52 patients with ESCC were stained immunohistochemically,
it was found that Mina53 was highly expressed in 83% of specimens. Anti-Mina53 antibody stained tumors more efficiently than
antibody against Ki-67, a cell proliferation biomarker, in some cancer specimens. Patients with high expression of Mina53
had shorter survival periods, whereas the expression level of Ki-67 in ESCC showed no relationship to patient outcome.
Conclusions: Taken together, our results indicate that expression of Mina53 is a characteristic feature of ESCC and suggest that immunostaining
by anti-Mina53 antibody may be useful as a potential prognostic indicator. |
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ISSN: | 1078-0432 1557-3265 |
DOI: | 10.1158/1078-0432.CCR-03-0543 |