Cloning and characterisation of the immunophilin X-CypA in Xenopus laevis

This paper reports the cloning of Xenopus laevis, cyclophilin A gene, X-CypA. This study is the first developmental and functional characterisation in vivo of cyclophilin A in a vertebrate. X-CypA belongs to the superfamily of the immunophilin/PPIase proteins that can bind the immunosuppressant drug...

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Bibliographic Details
Published in:Gene Expression Patterns 2004-11, Vol.5 (1), p.51-60
Main Authors: Massé, Karine, Bhamra, Surinder, Haldin, Caroline E., Jones, Elizabeth A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cyclophilin A
Cyclophilin A - genetics
Cyclophilin A - metabolism
DNA, Complementary
Immunophilin/PPIase
In Situ Hybridization
Molecular Sequence Data
Neural crest
Neural Crest - abnormalities
Neural Crest - embryology
Neural Crest - enzymology
Phenotype
Pronephros
Sequence Alignment
Xenopus laevis
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Summary:This paper reports the cloning of Xenopus laevis, cyclophilin A gene, X-CypA. This study is the first developmental and functional characterisation in vivo of cyclophilin A in a vertebrate. X-CypA belongs to the superfamily of the immunophilin/PPIase proteins that can bind the immunosuppressant drug Cyclosporin A. Sequence analysis showed that X-CypA is highly conserved during evolution. RT-PCR and in situ hybridisation analysis showed that X-CypA expression is regulated during development and its transcripts are found in three major expression domains: nervous system, sensory organs and pronephros. Over-expression of X-CypA in embryos, analysed by in situ hybridisation and RT-PCR, leads to an expansion and disorganisation of the neural crest domain.
ISSN:1567-133X
1872-7298
DOI:10.1016/j.modgep.2004.06.007