Identification of the Luciferin-Luciferase System and Quantification of Coelenterazine by Mass Spectrometry in the Deep-Sea Luminous Ostracod Conchoecia pseudodiscophora

The bioluminescence system of the ostracod Conchoecia pseudodiscophora, which is abundant in the Sea of Japan, has been characterized. The luminescence (λmax=463 nm) is produced by a luciferin-luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramid...

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Published in:Chembiochem : a European journal of chemical biology 2004-11, Vol.5 (11), p.1495-1499
Main Authors: Oba, Yuichi, Tsuduki, Hiroshi, Kato, Shin-ichi, Ojika, Makoto, Inouye, Satoshi
Format: Article
Language:English
Subjects:
Animals
coelenteramide
coelenteramine
Crustacea - chemistry
Cypridina luciferin
Firefly Luciferin - chemistry
Firefly Luciferin - metabolism
Imidazoles - analysis
Imidazoles - chemistry
Imidazoles - metabolism
Luciferases - chemistry
Luciferases - metabolism
luminescence
Luminescent Measurements
mass spectrometry
Mass Spectrometry - methods
Molecular Structure
Pyrazines - analysis
Pyrazines - chemistry
Pyrazines - metabolism
Species Specificity
Time Factors
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Summary:The bioluminescence system of the ostracod Conchoecia pseudodiscophora, which is abundant in the Sea of Japan, has been characterized. The luminescence (λmax=463 nm) is produced by a luciferin-luciferase reaction, and the luciferin has been identified as coelenterazine. Coelenterazine, coelenteramide, and coelenteramine from C. pseudodiscophora were quantified by LC-ESI-MS/MS analysis. The coelenterazine content was estimated to be approximately 230 pg per animal by using a calibration curve of synthetic coelenterazine. The reaction between homogenates of C. pseudodiscophora and synthetic coelenterazine showed luminescence activity; this suggests that a coelenterazine-type luciferase is present.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200400102