Analysis of plant extracts by NIRS: simultaneous determination of kavapyrones and water in dry extracts of Piper methysticum Forst

• Published in: Journal of pharmaceutical and biomedical analysis 2004-11, Vol.36 (4), p.859-864
• Main Authors: Gaub, M., Roeseler, Ch, Roos, G., Kovar, K.-A.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General pharmacology; Kava; Medical sciences; NIR spectroscopy; Partials least squares 2 (PLS 2); Pharmacology. Drug treatments; Piper methysticum Forst; Plant Extracts - analysis; Pyrones - analysis; Routine quality control; Spectroscopy, Near-Infrared - methods; Water - analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2004.06.030

A near-infrared reflection spectroscopy (NIRS) method was developed to determine the total content of kavapyrones, kavain and water in dry extracts of Piper methysticum Forst. (kava kava, Piperaceae). Based on the recorded spectra and the reference data, performed by HPLC and Karl Fischer titration, a chemometrical analysis was calculated using PLS 2 algorithm. In general, good calibration statistics are obtained for the prediction of the different contents presenting high correlation coefficients ( r 2 > 0.9913) and low root mean square errors of prediction (RMSEP < 0.094%). Usually the main water bands are “cut out” of the spectra to improve the model, however this is associated with the loss of relevant spectroscopic information. Thus, the entire spectrum including the OH bands is used, as these are not only found in water but also in the kavapyrones. The use of this new strategy succeeds in overcoming the difficulties in NIRS and establishes NIRS as a valid alternative in the routine quality control of plant extracts.