Improvement of a latex agglutination test for the evaluation of oxacillin resistance in coagulase-negative staphylococci

The “Slidex MRSA Detection” test (Denka Seiken, Japan) is a latex agglutination assay able to detect PBP2a. We evaluated its ability to differentiate mecA-positive from mecA-negative coagulase-negative staphylococci. We included 100 coagulase-negative staphylococci clinical isolates belonging to 9 s...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2004-11, Vol.50 (3), p.223-225
Main Authors: Corso, A., Soloaga, R., Faccone, D., Gagetti, P., Corbella, S., Iglesias, M., Galas, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Infectious diseases
Latex Fixation Tests - methods
Medical sciences
Microbiology
Oxacillin - pharmacology
Penicillin Resistance - genetics
Penicillin-Binding Proteins - analysis
Reagent Kits, Diagnostic - microbiology
Sensitivity and Specificity
Species Specificity
Staphylococcus - classification
Staphylococcus - drug effects
Staphylococcus - genetics
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Summary:The “Slidex MRSA Detection” test (Denka Seiken, Japan) is a latex agglutination assay able to detect PBP2a. We evaluated its ability to differentiate mecA-positive from mecA-negative coagulase-negative staphylococci. We included 100 coagulase-negative staphylococci clinical isolates belonging to 9 species, 54 mecA positive and 46 mecA negative, as characterized by PCR. The specificity achieved using the manufacturer's instructions was 100%, but the sensitivity was only 57%. To increase sensitivity, we introduced modifications into the standard protocol. Using either large inocula or oxacillin induction before test performance, we achieved 100% sensitivity.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2004.06.005