Quantitative determination of alpha(s2)- and alpha(s1)-casein in goat's milk with different genotypes by capillary electrophoresis

A capillary electrophoresis (CE) method has been applied for the quantitative determination of alpha(s1)- and alpha(s2)-CN in goat's milk. Several analytical parameters were evaluated showing the reliability of this CE method. Coefficients of determination (R2) greater than 99% were obtained an...

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Bibliographic Details
Published in:Journal of chromatography. A 2004-10, Vol.1054 (1-2), p.279-284
Main Authors: Gómez-Ruiz, José Angel, Miralles, Beatriz, Agüera, Pastora, Amigo, Lourdes
Format: Article
Language:English
Subjects:
Animals
Caseins - analysis
Electrophoresis, Capillary - methods
Genotype
Goats
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Summary:A capillary electrophoresis (CE) method has been applied for the quantitative determination of alpha(s1)- and alpha(s2)-CN in goat's milk. Several analytical parameters were evaluated showing the reliability of this CE method. Coefficients of determination (R2) greater than 99% were obtained and determination limits of 1.23 and 0.98 mg/ml were achieved for alpha(s1)- and alpha(s2)-CN, respectively. The analytical parameters studied in terms of accuracy, precision and recovery were within acceptable limits. Among 18 samples of 4 different genotypes (BB, EE, BF and FF) for alpha(s1)-CN were analysed, different amounts were obtained from the genotypes.
ISSN:0021-9673
DOI:10.1016/j.chroma.2004.07.089