Inhibitory Effects of Human and Bovine Milk Constituents on Rotavirus Infections

• Published in: Journal of dairy science 2004-12, Vol.87 (12), p.4088-4096
• Main Authors: Kvistgaard, A.S., Pallesen, L.T., Arias, C.F., López, S., Petersen, T.E., Heegaard, C.W., Rasmussen, J.T.
• Format: Article
• Language: English
• Subjects: Animal productions; Animals; Antigens, Surface - immunology; antiviral proteins; Biological and medical sciences; Bovine rotavirus; Breast Feeding; breast milk; Caco-2 Cells - virology; Cattle; Cell Line - virology; cell membranes; Child, Preschool; fat globules; Food industries; Fundamental and applied biological sciences. Psychology; Human rotavirus; Humans; Infant; lactoferrin; Milk - chemistry; Milk and cheese industries. Ice creams; milk fat globule membrane protein; Milk Proteins - immunology; Milk, Human - chemistry; Mucin-1 - immunology; Peptide Fragments - immunology; rotavirus; Rotavirus Infections - immunology; Rotavirus Infections - prevention & control; Terrestrial animal productions; Vertebrates; whey protein
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(04)73551-1

Among etiologic agents, rotavirus is the major cause of severe dehydration diarrhea in infant mammals. In vitro and in vivo studies have indicated that the human milk-fat globule protein lactadherin inhibits rotavirus binding and protects breast-fed children against symptomatic rotavirus infection. The present work was conducted to evaluate the effect of lactadherin, along with some other milk proteins and fractions, on rotavirus infections in MA104 and Caco-2 cell lines. It is shown that human, and not bovine, lactadherin inhibits Wa rotavirus infection in vitro. Human lactadherin seems to act through a mechanism involving protein-virus interactions. The reason for the activity of human lactadherin is not clear, but it might lie within differences in the protein structure or the attached oligosaccharides. Likewise, in our hands, bovine lactoferrin did not show any suppressive activity against rotavirus. In contrast, MUC1 from bovine milk inhibits the neuraminidase-sensitive rotavirus RRV strain efficiently, whereas it has no effect on the neuraminidase-resistant Wa strain. Finally, a bovine macromolecular whey protein fraction turned out to have an efficient and versatile inhibitory activity against rotavirus.