Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing

The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a product...

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Published in:Trends in biotechnology (Regular ed.) 2009-04, Vol.27 (4), p.240-247
Main Authors: Azevedo, Ana M, Rosa, Paula A.J, Ferreira, I. Filipa, Aires-Barros, M. Raquel
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - isolation & purification
Biological and medical sciences
Biopharmaceutics - methods
Biotechnology
Cell culture
Chemical Fractionation - methods
Cost reduction
Fundamental and applied biological sciences. Psychology
Growth factors
Hormones
Human Growth Hormone - isolation & purification
Humans
Insulin - isolation & purification
Insulin-Like Growth Factor Binding Protein 1 - isolation & purification
Internal Medicine
Methods. Procedures. Technologies
Molecular weight
Others
Pharmaceutical industry
Pharmaceutical Preparations - isolation & purification
Polymers
Polymers - chemistry
Process controls
Productivity
Proteins
Various methods and equipments
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description The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a production bottleneck that is shifting the costs of production. This review focuses on the use of aqueous two-phase extraction as an option for the downstream processing of therapeutic proteins. It is a potential and promising liquid–liquid extraction technique for the purification of biomolecules, such as monoclonal antibodies, growth factors and hormones, that combines a high selectivity and biocompatibility with an easy scale-up and continuous operation mode.
doi_str_mv 10.1016/j.tibtech.2009.01.004
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subjects Antibodies, Monoclonal - isolation & purification
Biological and medical sciences
Biopharmaceutics - methods
Biotechnology
Cell culture
Chemical Fractionation - methods
Cost reduction
Fundamental and applied biological sciences. Psychology
Growth factors
Hormones
Human Growth Hormone - isolation & purification
Humans
Insulin - isolation & purification
Insulin-Like Growth Factor Binding Protein 1 - isolation & purification
Internal Medicine
Methods. Procedures. Technologies
Molecular weight
Others
Pharmaceutical industry
Pharmaceutical Preparations - isolation & purification
Polymers
Polymers - chemistry
Process controls
Productivity
Proteins
Various methods and equipments
title Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing
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