Plant Rho-type (Rop) GTPase-dependent activation of receptor-like cytoplasmic kinases in vitro

Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly an...

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Bibliographic Details
Published in:FEBS letters 2009-04, Vol.583 (7), p.1175-1182
Main Authors: Dorjgotov, Dulguun, Jurca, Manuela E., Fodor-Dunai, Csilla, Szűcs, Attila, Ötvös, Krisztina, Klement, Éva, Bíró, Judit, Fehér, Attila
Format: Article
Language:English
Subjects:
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Enzyme Activation - physiology
Kinase activity
Medicago sp
Medicago truncatula - enzymology
Medicago truncatula - genetics
Protein Kinases - genetics
Protein Kinases - metabolism
Protein phosphorylation
Protein–protein interaction
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
rho GTP-Binding Proteins - genetics
rho GTP-Binding Proteins - metabolism
Signal transduction
Small GTP-binding protein
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Summary:Plants have evolved distinct mechanisms to link Rho-type (Rop) GTPases to downstream signaling pathways as compared to other eukaryotes. Here, experimental data are provided that members of the Medicago, as well as Arabidopsis, receptor-like cytoplasmic kinase family (RLCK Class VI) were strongly and specifically activated by GTP-bound Rop GTPases in vitro. Deletion analysis indicated that the residues implicated in the interaction might be distributed on various parts of the kinases. Using a chimaeric Rop GTPase protein, the importance of the Rho-insert region in kinase activation could also be verified. These data strengthen the possibility that RLCKs may serve as Rop GTPase effectors in planta.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2009.02.047