Application of SEC-ICP-MS for comparative analyses of metal-containing species in cancerous and healthy human thyroid samples

Size exclusion chromatography (SEC) was coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for speciation study of trace metals in cancerous thyroid tissues in comparison to healthy thyroids aimed to estimation of changes in metalloprotein speciation in pathological tissue. The...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2004-09, Vol.380 (2), p.198-203
Main Authors: Boulyga, Sergei F, Loreti, Valeria, Bettmer, Jörg, Heumann, Klaus G
Format: Article
Language:English
Subjects:
cadmium
Chromatography, Gel - methods
copper
gel chromatography
Humans
In Vitro Techniques
lead
mass spectrometry
Mass Spectrometry - methods
metals
Metals - analysis
monitoring
thyroid gland
Thyroid Gland - chemistry
Thyroid Neoplasms - chemistry
Trace Elements - analysis
zinc
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Summary:Size exclusion chromatography (SEC) was coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for speciation study of trace metals in cancerous thyroid tissues in comparison to healthy thyroids aimed to estimation of changes in metalloprotein speciation in pathological tissue. The study showed a presence of species binding Cu, Zn, Cd and Pb in healthy thyroid tissue with a good reproducibility of chromatographic results, whereas the same species could not be detected in cancerous tissues. Thus, remarkable differences with respect to metal-binding species were revealed between healthy and pathological thyroid samples, pointing out a completely different distribution of trace metals in cancerous tissues. The metal-binding species could not be identified in the frame of this work because of a lack of appropriate standards. Nevertheless, the results obtained confirm the suitability of SEC-ICP-MS for monitoring of changes in trace metal distribution in cancerous tissue and will help to better understand the role of metal-containing species in thyroid pathology.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-004-2699-6