Zwitterionic molecularly imprinted polymer-based solid-phase micro-extraction coupled with molecularly imprinted polymer sensor for ultra-trace sensing of L-histidine

The proposed L‐histidine sensing system composed of a molecularly imprinted solid‐phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non‐specifi...

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Bibliographic Details
Published in:Journal of separation science 2009-04, Vol.32 (7), p.1096-1105
Main Authors: Prasad, Bhim Bali, Tiwari, Khushaboo, Singh, Meenakshi, Sharma, Piyush S., Patel, Amit K., Srivastava, Shrinkhala
Format: Article
Language:English
Subjects:
Biological and medical sciences
Histidine - analysis
Human blood serum
Investigative techniques, diagnostic techniques (general aspects)
Ions - chemistry
L-Histidine
Medical sciences
Microscopy, Electron, Scanning
Miscellaneous. Technology
Molecular Imprinting - instrumentation
Molecular Imprinting - methods
Molecular Structure
Molecularly imprinted polymer brush
Molecularly imprinted polymer-sensor
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymers - chemistry
Sensitivity and Specificity
Solid Phase Microextraction - instrumentation
Solid Phase Microextraction - methods
Solid-phase microextraction
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Summary:The proposed L‐histidine sensing system composed of a molecularly imprinted solid‐phase microextraction component combined with a molecularly imprinted polymer sensor was used to determine critical levels of test analyte in a complex matrix of highly diluted human blood serum without any non‐specific sorption and false‐positive contributions. The molecularly imprinted polymer was a zwitterionic polymer brush derived from the disodium salt of EDTA and chloranil, grafted to solid‐phase microextraction material. The hyphenated approach was able to detect L‐histidine quantitatively with a limit of detection as low as 0.0435 ng/mL (RSD = 0.2%, S/N = 3).
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200800595