Establishment of T cell-specific and natural killer cell-specific unigene sets: towards high-throughput genomics of leukaemia

Summary We report the establishment of highly non‐redundant unigene sets consisting of cDNA clones derived from T lymphocytes and natural killer cells. Each set consists of 10 506 and 13 409 clones, respectively, arrayed on nylon membranes in duplicate. The sets provide an excellent tool for genome‐...

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Bibliographic Details
Published in:European journal of immunogenetics 2004-12, Vol.31 (6), p.253-257
Main Authors: Illiger, J., Herwig, R., Steinfath, M., Przewieslik, T., Elge, T., Bull, Ch, Radelof, U., Lehrach, H., Janitz, M.
Format: Article
Language:English
Subjects:
Blotting, Northern
DNA, Complementary - metabolism
Gene Expression - physiology
Gene Expression Profiling
Gene Library
Humans
Jurkat Cells
Killer Cells, Natural - metabolism
Leukemia - genetics
Leukemia - metabolism
Male
T-Lymphocytes - metabolism
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Summary:Summary We report the establishment of highly non‐redundant unigene sets consisting of cDNA clones derived from T lymphocytes and natural killer cells. Each set consists of 10 506 and 13 409 clones, respectively, arrayed on nylon membranes in duplicate. The sets provide an excellent tool for genome‐wide gene expression analysis studies in immunology research.
ISSN:0960-7420
1365-2370
DOI:10.1111/j.1365-2370.2004.00483.x