Elicitor effects on Penicillium chrysogenum morphology in submerged cultures

Changes in morphology and sporulation were investigated in liquid cultures of Penicillium chrysogenum P2 supplemented with carbohydrate oligosaccharides. Sodium alginate and locust‐bean (Ceratonia siliqua) gum‐derived oligosaccharides were used as elicitors. Spore germination was inhibited by the ad...

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Bibliographic Details
Published in:Biotechnology and applied biochemistry 2004-12, Vol.40 (3), p.229-233
Main Authors: Radman, Romeo, Bucke, Christopher, Keshavarz, Tajalli
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cell Culture Techniques - methods
Cell Proliferation - drug effects
Cell Size - drug effects
Ceratonia siliqua
elicitor
Fundamental and applied biological sciences. Psychology
Hyphae - drug effects
Hyphae - physiology
Hyphae - ultrastructure
liquid culture
morphology
oligosaccharide
Oligosaccharides - pharmacology
Penicillium chrysogenum
Penicillium chrysogenum - cytology
Penicillium chrysogenum - drug effects
Penicillium chrysogenum - physiology
Spores, Fungal - drug effects
Spores, Fungal - growth & development
Spores, Fungal - ultrastructure
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Summary:Changes in morphology and sporulation were investigated in liquid cultures of Penicillium chrysogenum P2 supplemented with carbohydrate oligosaccharides. Sodium alginate and locust‐bean (Ceratonia siliqua) gum‐derived oligosaccharides were used as elicitors. Spore germination was inhibited by the addition of OG (oligoguluronate) elicitor (30% inhibition when compared with control). Addition of any of the elicitors to stirred‐tank cultures increased hyphal‐tip numbers, clump area and spore counts. MO (mannan oligosaccharide) had the greatest effect on the parameters studied, followed by OM (oligomannuronate) and OG. Hyphal‐tip numbers increased by 19, 29 and 47% with OG, OM and MO respectively. Average clump area in the presence of OG, OM and MO increased by 23, 32 and 59% respectively. A notable increase in spore numbers was observed in all the supplemented stirred‐tank reactor cultures, with MO showing the highest enhancement.
ISSN:0885-4513
1470-8744
DOI:10.1042/BA20040062