Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF

BACKGROUND: A biochemical marker for embryo development would increase the chance of a successful pregnancy with IVF by optimizing oocyte and embryo selection, and allow fewer embryos to be transferred. In this study, we correlated cumulus granulosa cell gene expression of hyaluronic acid synthase 2...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2004-12, Vol.19 (12), p.2869-2874
Main Authors: McKenzie, L.J., Pangas, S.A., Carson, S.A., Kovanci, E., Cisneros, P., Buster, J.E., Amato, P., Matzuk, M.M.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Birth control
C-Reactive Protein
Cyclooxygenase 2
embryo selection
Embryo, Mammalian - physiology
Female
Fertilization in Vitro
Gene Expression
Glucuronosyltransferase - genetics
Granulosa Cells - physiology
growth differentiation factor 9
Gynecology. Andrology. Obstetrics
Humans
Hyaluronan Synthases
Intercellular Signaling Peptides and Proteins - genetics
Medical sciences
Membrane Proteins
oocyte-regulated genes
Predictive Value of Tests
Pregnancy
Pregnancy Rate
Prostaglandin-Endoperoxide Synthases - genetics
reproductive success
ROC Curve
Serum Amyloid P-Component
Sterility. Assisted procreation
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Summary:BACKGROUND: A biochemical marker for embryo development would increase the chance of a successful pregnancy with IVF by optimizing oocyte and embryo selection, and allow fewer embryos to be transferred. In this study, we correlated cumulus granulosa cell gene expression of hyaluronic acid synthase 2 (HAS2), cyclooxygenase 2 (COX2; PTGS2) and gremlin (GREM1) with subsequent embryo development in search of a parameter for embryo selection. METHODS: Cumulus cell gene expression was determined prospectively on eight consecutive patients undergoing IVF with ICSI. Immediately following oocyte retrieval, the cumulus was stripped from the oocyte, and cumulus gene expression for PTGS2, HAS2 and GREM1 was assessed using a one-step real-time quantitative RT–PCR assay. Oocyte quality, fertilization and embryo morphology were correlated to relative gene expression. RESULTS: Gene expression data were available on cumulus cells from 108 oocytes that developed into 70 embryos (64.8% fertilization rate). Cumulus PTGS2, HAS2 and GREM1 expression was higher from oocytes that developed into higher quality embryos (grades 3, 4 and 5) compared with lower quality embryos (grades 1 and 2) (P
ISSN:0268-1161
1460-2350
DOI:10.1093/humrep/deh535