Isolation of ESTs from cacao (Theobroma cacao L.) leaves treated with inducers of the defense response

Pathogenic diseases represent a major constraint to the growth and yield of cacao (Theobroma cacao L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated by endogenous signaling molecules such as salicylic acid, jasmonic acid an...

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Bibliographic Details
Published in:Plant cell reports 2004-11, Vol.23 (6), p.404-413
Main Authors: Verica, J.A, Maximova, S.N, Strem, M.D, Carlson, J.E, Bailey, B.A, Guiltinan, M.J
Format: Article
Language:English
Subjects:
benzothiadiazole
Bioinformatics
Biological and medical sciences
Biotechnology
Cacao - genetics
Cacao - metabolism
complementary DNA
disease resistance
DNA libraries
Eukaryotic cell cultures
Expressed Sequence Tags
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
fungal proteins
Fusarium oxysporum
Fusarium oxysporum f. sp. erythroxyli
gene expression regulation
Gene Expression Regulation, Plant
Gene Library
Hybridization
Methods. Procedures. Technologies
methyl jasmonate
Miscellaneous
Plant cells and fungal cells
Plant Diseases - genetics
Plant Leaves - metabolism
plant response
resistance mechanisms
salicylic acid
Seedlings - metabolism
sequence analysis
Signal Transduction
suppressive subtractive hybridization
Theobroma cacao
transcription (genetics)
Transcriptional Activation
Up-Regulation
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Summary:Pathogenic diseases represent a major constraint to the growth and yield of cacao (Theobroma cacao L.). Ongoing research on model plant systems has revealed that defense responses are activated via signaling pathways mediated by endogenous signaling molecules such as salicylic acid, jasmonic acid and ethylene. Activation of plant defenses is associated with changes in the expression of large numbers of genes. To gain a better understanding of defense responses in cacao, we have employed suppressive subtractive hybridization (SSH) cDNA libraries, macroarray hybridization analysis, high throughput DNA sequencing and bioinformatics to identify cacao genes induced by these signaling molecules. Additionally, we investigated gene activation by a phytotoxic elicitor-like protein, Nep1. We have identified a unigene set of 1,256 members, including 330 members representing genes induced during the defense response.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0852-5