metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis Georgi) genotypes: discovery of novel compounds

In vitro manipulation of plant regeneration in the Chinese medicinal species Scutellaria baicalensis Georgi (Huang-qin) resulted in 26 chemically distinct germplasm lines. Antioxidant potential, growth rate and concentration of baicalin, baicalein, melatonin, and wogonin were the selective markers u...

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Bibliographic Details
Published in:Plant cell reports 2004-11, Vol.23 (6), p.419-425
Main Authors: Murch, S.J, Rupasinghe, H.P.V, Goodenowe, D, Saxena, P.K
Format: Article
Language:English
Subjects:
Antioxidants - analysis
bacalein
bacalin
Biological and medical sciences
Biotechnology
chemical constituents of plants
Drugs, Chinese Herbal - analysis
Eukaryotic cell cultures
Flavanones - analysis
Flavonoids - analysis
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genotypes
Hypericum perforatum
medicinal plants
Melatonin - analysis
Metabolites
metabolome
metabolomics
Methods. Procedures. Technologies
Miscellaneous
Plant cells and fungal cells
plant regeneration
Scutellaria baicalensis
Scutellaria baicalensis - chemistry
Scutellaria baicalensis - genetics
Scutellaria baicalensis - metabolism
wogonin
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Summary:In vitro manipulation of plant regeneration in the Chinese medicinal species Scutellaria baicalensis Georgi (Huang-qin) resulted in 26 chemically distinct germplasm lines. Antioxidant potential, growth rate and concentration of baicalin, baicalein, melatonin, and wogonin were the selective markers used to identify elite lines. Metabolomic analysis of a subset of the most distinct lines revealed that Huang-qin extracts contained over 2,000 compounds including 781 determined to be of putative medicinal importance as determined by a database search, as well as previously unidentified amino-derivatives of baicalin and wogonin. Huang-qin also contained a metabolite with the same net formula as hyperforin, previously thought to be unique to Hypericum perforatum L. Together these results provide new insights into the biochemical complexity of an important medicinal species and demonstrate the power of in vitro manipulation in combination with untargeted metabolomic screening for the production of new germplasm.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0862-3