Cryptic species in the Terfezia boudieri complex

Phylogenetic analyses have corroborated the discovery of three internal transcribed spacer (ITS) Types in Terfezia boudieri isolates in the course of earlier studies and have emphasized the divergence of Type 2 from Types 1 and 3. The application of molecular and physiological tools described below,...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2009-05, Vol.95 (4), p.351-362
Main Authors: Ferdman, Yael, Sitrit, Yaron, Li, Yong-Fang, Roth-Bejerano, Nurit, Kagan-Zur, Varda
Format: Article
Language:English
Subjects:
Amplified Fragment Length Polymorphism Analysis
Ascomycota - classification
Ascomycota - isolation & purification
Biological and medical sciences
Biomedical and Life Sciences
Chitin
Chitin Synthase - genetics
Cryptic species
DNA Fingerprinting
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Fungal Proteins
Genes
Genes, rRNA
Genotype
Life Sciences
Medical Microbiology
Microbiology
Molecular biology
Molecular Sequence Data
Mycelium - growth & development
Mycological Typing Techniques
Mycology
Mycorrhizae - growth & development
Original Paper
Phylogeny
Physiology
Plant Sciences
Proteins
RNA, Fungal - genetics
RNA, Ribosomal - genetics
Sequence Analysis, DNA
Soil Science & Conservation
Studies
Systematics
Tubulin - genetics
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Summary:Phylogenetic analyses have corroborated the discovery of three internal transcribed spacer (ITS) Types in Terfezia boudieri isolates in the course of earlier studies and have emphasized the divergence of Type 2 from Types 1 and 3. The application of molecular and physiological tools described below, revealed the existence of cryptic species within T. boudieri. The markers used include sequences taken from the 5' end of the ribosomal large subunit gene, a chitin synthase partial sequence, β-tubulin partial sequence and amplified fragment length polymorphism (AFLP)-based markers. Following initial sequencing of a single PCR amplified sample for each Type, mass analysis of specimens relied on RFLP differences between the Types. Over 100 fruit bodies, 30 or more specimens for each ITS Type, were tested with each of the markers. The markers analysis divided the isolates into three groups, each correlated to a specific ITS Type. Two of the physiological traits examined: mycelial proliferation and mycorrhiza formation, consistently showed responses paralleling the ITS Types; the data presented suggest that T. boudieri is comprised of three cryptic species.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-009-9321-z