Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis

Abstract The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, iso...

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Published in:FEMS microbiology letters 2004-12, Vol.241 (1), p.57-65
Main Authors: Moran, Anthony P., Shiberu, Bethlehem, Ferris, John A., Knirel, Yuriy A., Senchenkova, Sof'ya N., Perepelov, Andreij V., Jansson, Per-Erik, Goldberg, Joanna B.
Format: Article
Language:English
Subjects:
Core oligosaccharide
Epitopes
Genes
Genes, Bacterial - physiology
Genomes
Glucosyltransferase
Glucosyltransferases - genetics
Glycosyltransferase
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - metabolism
Homology
Immunoblotting
Lewis antigen
Lipopolysaccharide
Lipopolysaccharides
Lipopolysaccharides - biosynthesis
Lipopolysaccharides - chemistry
Microbiology
Structural analysis
Synthesis
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Summary:Abstract The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an α(1,2/3)-glucosyltransferase whereas HP1416 encodes an α(1,2/4)-glucosyltransferase.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.10.004