Aberrant Methylation of the p16 Gene Is Frequently Detected in Advanced Colorectal Cancer

Background: A tumor suppressor gene, p16, was found to harbor promoter hypermethylation associated with the loss of protein expression in cancer cells, suggesting that p16 inactivation due to promoter methylation was important for colorectal tumorigenesis. Materials and Methods: The methylation stat...

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Bibliographic Details
Published in:Anticancer research 2009-01, Vol.29 (1), p.275-277
Main Authors: GOTO, Tetsuhiro, MIZUKAMI, Hiroki, SHIRAHATA, Atsushi, SAKATA, Makiko, SAITO, Mitsuo, ISHIBASHI, Kazuyoshi, KIGAWA, Gaku, NEMOTO, Hiroshi, SANADA, Yutaka, HIBI, Kenji
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
DNA Methylation
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genes, p16
Humans
Male
Medical sciences
Middle Aged
Neoplasm Staging
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Young Adult
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Summary:Background: A tumor suppressor gene, p16, was found to harbor promoter hypermethylation associated with the loss of protein expression in cancer cells, suggesting that p16 inactivation due to promoter methylation was important for colorectal tumorigenesis. Materials and Methods: The methylation status of the p16 gene was examined in primary carcinomas and the corresponding normal tissues derived from 50 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated. Results: Aberrant methylation of the p16 gene was detected in 20 out of the 50 (40%) primary colon carcinomas, suggesting that the aberrant methylation of p16 was frequently observed in colorectal carcinomas. The clinicopathological data were then correlated with these results. Significant differences were observed with Dukes' stage (p=0.0495) and lymphatic invasion (p=0.0277). Conclusion: p16 might act as a tumor suppressor in colorectal carcinomas and was more frequently methylated in advanced colorectal carcinomas.
ISSN:0250-7005
1791-7530