Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene

Microbial production of butanediol and acetoin has received increasing interest because of their diverse potential practical uses. Although both products are fermentative in nature, their optimal production requires a low level of oxygen. In this study, the use of a recombinant oxygen uptake system...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2004-10, Vol.26 (5), p.325-330
Main Authors: Geckil, Hikmet, Barak, Ze'ev, Chipman, David M, Erenler, Sebnem O, Webster, Dale A, Stark, Benjamin C
Format: Article
Language:English
Subjects:
Acetoin - metabolism
Acid production
Aerogenes
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bioreactors - microbiology
Butylene Glycols - metabolism
Enterobacter aerogenes
Enterobacter aerogenes - genetics
Enterobacter aerogenes - metabolism
Fermentation
Genetic Enhancement - methods
Hemoglobins - genetics
Hemoglobins - metabolism
Metabolites
Oxygen uptake
Protein Engineering - methods
Recombinant Proteins - metabolism
Truncated Hemoglobins
Vitreoscilla
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Summary:Microbial production of butanediol and acetoin has received increasing interest because of their diverse potential practical uses. Although both products are fermentative in nature, their optimal production requires a low level of oxygen. In this study, the use of a recombinant oxygen uptake system on production of these metabolites was investigated. Enterobacter aerogenes was transformed with a pUC8-based plasmid carrying the gene (vgb) encoding Vitreoscilla (bacterial)hemoglobin (VHb). The presence of vgb and production of VHb by this strain resulted in an increase in viability from 72 to 96 h in culture, but no overall increase in cell mass. Accumulation of the fermentation products acetoin and butanediol were enhanced (up to 83%) by the presence of vgb/VHb. This vgb/VHb related effect appears to be due to an increase of flux through the acetoin/butanediol pathway, but not at the expense of acid production.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-004-0373-1