Simple and Rapid Procedure Suitable for Quantitative Isolation of Low and High Molecular Weight Extracellular Nucleic Acids

The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respect...

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Bibliographic Details
Published in:Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2004-10, Vol.23 (6-7), p.873-877
Main Authors: Tamkovich, Svetlana N., Laktionov, Pavel P., Rykova, Elena Yu, Vlassov, Valentin V.
Format: Article
Language:English
Subjects:
Circulating nucleic acids
Electrophoresis, Agar Gel
Isolation of DNA
Isolation of RNA
Molecular Weight
Nucleic Acids - chemistry
Nucleic Acids - isolation & purification
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Summary:The procedure based on binding of nucleic acids with glass surface in presence of chaotropic salts was adapted for efficient isolation of 100-10000 b.p. DNA fragments and 50-10,000 b. RNA fragments. The method provide 90% and 85% efficacy of isolation of 100 b.p. DNA and 100 b. RNA fragments respectively. High molecular weight nucleic acids are isolated with 98% efficacy. Isolated nucleic acids are free from contaminations, influencing nucleic acids modifying enzymes and fluorochromes. The method is rapid, simple and cost-effective.
ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-200026034