Nucleolar Size and Activity Are Related to pRb and p53 Status in Human Breast Cancer

Cell proliferation is tightly coordinated with cell growth. The oncosuppressor proteins pRb and p53 may exert a key role in coupling growth and proliferation by controlling both ribosome biogenesis and cell cycle progression. In the present study we evaluated the relationship between the pRb and p53...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 2004-12, Vol.52 (12), p.1601-1607
Main Authors: Trere, Davide, Ceccarelli, Claudio, Montanaro, Lorenzo, Tosti, Elena, Derenzini, Massimo
Format: Article
Language:English
Subjects:
Breast Neoplasms - metabolism
Breast Neoplasms - ultrastructure
Female
Humans
Image Cytometry
Image Processing, Computer-Assisted
Immunohistochemistry
Nucleolus Organizer Region - metabolism
Nucleolus Organizer Region - ultrastructure
Retinoblastoma Protein - biosynthesis
Ribosomes - metabolism
Ribosomes - ultrastructure
Tumor Suppressor Protein p53 - biosynthesis
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Summary:Cell proliferation is tightly coordinated with cell growth. The oncosuppressor proteins pRb and p53 may exert a key role in coupling growth and proliferation by controlling both ribosome biogenesis and cell cycle progression. In the present study we evaluated the relationship between the pRb and p53 status and rRNA transcriptional activity in histological sections of 343 human primary breast carcinomas. Ribosomal biogenesis was quantified by morphometric analysis of silver-stained interphase nucleolar organizer regions (AgNORs). pRb and p53 status was assessed by immunohistochemistry. Twenty-four tumors were considered to be pRb deleted, 260 tumors showed a phosphorylated-pRb labeling index (LI) up to 25%, and 55 tumors an LI >25%. Tumors with deleted pRb or phosphory-lated-pRb-LI ×25% were characterized by significantly greater mean AgNOR area values than those with unaltered pRb (p
ISSN:0022-1554
1551-5044
DOI:10.1369/jhc.4A6454.2004