CCL22 Recruits CD4-positive CD25-positive Regulatory T Cells into Malignant Pleural Effusion

Purpose: The aim of this study was to explore the presence of the chemokines CCL22 and CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural space. Experimental Design: T...

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Bibliographic Details
Published in:Clinical cancer research 2009-04, Vol.15 (7), p.2231-2237
Main Authors: Qin, Xue-Jun, Shi, Huan-Zhong, Deng, Jing-Min, Liang, Qiu-Li, Jiang, Jing, Ye, Zhi-Jian
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antineoplastic agents
Biological and medical sciences
Chemokine CCL17 - analysis
Chemokine CCL22 - metabolism
Chemokine CCL22 - pharmacology
Chemokine CCL22 - physiology
Chemotaxis
Humans
Interleukin-2 Receptor alpha Subunit - analysis
lung cancer
malignant pleural effusion
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Pleural Effusion, Malignant - immunology
regulatory T cells
T-Lymphocytes, Regulatory - immunology
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Summary:Purpose: The aim of this study was to explore the presence of the chemokines CCL22 and CCL17 in malignant pleural effusion, and the chemoattractant activity of these chemokines on CD4-positive CD25-positive Foxp3-positive regulatory T cells infiltrating into the pleural space. Experimental Design: The concentrations of CCL22 and CCL17 in both pleural effusions and sera from 33 patients with lung cancer were determined. Flow cytometry was done to determine T lymphocyte subsets in cell pellets of pleural effusion. Pleural cells were analyzed for the expression of CCL22 and CCL17. The chemoattractant activity of CCL22 for regulatory T cells in vitro and in vivo was also observed. Results: The concentration of CCL22 in malignant pleural effusion was significantly higher than that in the corresponding serum. Pleural fluid from lung cancer patients was chemotactic for regulatory T cells, and this activity was partly blocked by an anti-CCL22, but not by an anti-CCL17 antibody. Intrapleural administration of CCL22 of patients produced a marked progressive influx of regulatory T cells into pleural space. Conclusions: Compared with serum, CCL22 seemed to be increased in malignant pleural effusion, and could directly induce regulatory T cell infiltration into the pleural space in patients with malignant effusion.
ISSN:1078-0432
1557-3265
DOI:10.1158/1078-0432.CCR-08-2641