Hepatitis B virus enhances interleukin-27 expression both in vivo and in vitro

Abstract The immune system plays important roles in determining the outcomes of hepatitis B virus (HBV) infection. Interleukin-27 (IL-27) is a recently identified pro-inflammatory cytokine produced by macrophages, dendritic cells, and epithelial cells. To determine the correlation between HBV infect...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2009-04, Vol.131 (1), p.92-97
Main Authors: Zhu, Chengliang, Zhang, Rui, Liu, Li, Rasool, Sahibzada T, Mu, Yongxin, Sun, Wei, Hao, Qian, Liu, Fang, Zhu, Ying, Wu, Jianguo
Format: Article
Language:English
Subjects:
Acute Disease
Acute hepatitis B
Adult
Allergy and Immunology
Antigen Presentation
Biological and medical sciences
Cell Line
Chronic hepatitis B
Cytokines
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gastroenterology. Liver. Pancreas. Abdomen
HBV e antigen
Hepatitis B - immunology
Hepatitis B - virology
Hepatitis B e Antigens - blood
Hepatitis B e Antigens - immunology
Hepatitis B Surface Antigens - blood
Hepatitis B Surface Antigens - immunology
Hepatitis B virus
Hepatitis B virus - immunology
Hepatitis B, Chronic - blood
Hepatitis B, Chronic - immunology
Hepatitis B, Chronic - virology
Hepatocellular carcinoma
Human viral diseases
Humans
Infectious diseases
Interleukin-27
Interleukins - biosynthesis
Interleukins - blood
Interleukins - genetics
Interleukins - immunology
Liver cirrhosis
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Middle Aged
Monocytes
Other diseases. Semiology
Transfection
Tumors
Viral diseases
Viral hepatitis
Viral Load
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Summary:Abstract The immune system plays important roles in determining the outcomes of hepatitis B virus (HBV) infection. Interleukin-27 (IL-27) is a recently identified pro-inflammatory cytokine produced by macrophages, dendritic cells, and epithelial cells. To determine the correlation between HBV infection and IL-27 expression, we investigated the serum IL-27 levels in patients with HBV infection and in healthy individuals. Results showed that IL-27 was significantly elevated in patients as compared to healthy individuals ( P < 0.001). IL-27 was also detected at higher levels in patients with liver cirrhosis or hepatocellular carcinoma than those with acute hepatitis B or chronic hepatitis B ( P < 0.05). We also found that IL-27 expression was influenced by HBV e antigen. In addition, our in vitro studies demonstrated that IL-27 promoter activity, mRNA and protein expression were all stimulated in cells transfected with infectious HBV clone.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2008.10.011