Detection of stx1 , stx2 , and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction

Abstract We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1 , stx2 , and eae , without the use of probes. The method, which was validated on a collectio...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2009-05, Vol.64 (1), p.98-101
Main Authors: Chassagne, Laurent, Pradel, Nathalie, Robin, Frédéric, Livrelli, Valérie, Bonnet, Richard, Delmas, Julien
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - genetics
Child
eae
EHEC
Enterohemorrhagic Escherichia coli - genetics
Escherichia coli
Escherichia coli Infections - microbiology
Escherichia coli Proteins - genetics
Feces - microbiology
Humans
Infectious Disease
Internal Medicine
Organic Chemicals - metabolism
PCR
Polymerase Chain Reaction - methods
Real-time
Shiga Toxin 1 - genetics
Shiga Toxin 2 - genetics
Staining and Labeling
STEC
Stools
stx
SYBR Green
Virulence Factors - genetics
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Summary:Abstract We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1 , stx2 , and eae , without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2009.01.031