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Characterization of hepatic sexual dimorphism in Alb-DsRed2 transgenic rats
We previously created the Alb-DsRed2 transgenic (Tg) rat that specifically expresses the red fluorescent protein, DsRed2, in the liver. Herein, we demonstrate that the DsRed2 expression is sexually dimorphic and exhibits a male-specific pattern. The profiling of sexual dimorphism in DsRed2 expressio...
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| Published in: | Biochemical and biophysical research communications 2009-04, Vol.382 (1), p.46-50 |
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| Main Authors: | , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c451t-69117be59fd427c8629923ea627c3a35452af73d34691c2d39a2722dab85b4fb3 |
| container_end_page | 50 |
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| container_start_page | 46 |
| container_title | Biochemical and biophysical research communications |
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| creator | Arao, Yukitomo Hakamata, Yoji Igarashi, Yuka Sato, Yuki Kayama, Fujio Takahashi, Masafumi Kobayashi, Eiji Murakami, Takashi |
| description | We previously created the Alb-DsRed2 transgenic (Tg) rat that specifically expresses the red fluorescent protein, DsRed2, in the liver. Herein, we demonstrate that the DsRed2 expression is sexually dimorphic and exhibits a male-specific pattern. The profiling of sexual dimorphism in DsRed2 expression during pre-pubertal development was investigated using an
in vivo fluorescent imaging analysis. The DsRed2 expression decreased gradually in both sexes until 28
days after birth. While DsRed2 expression was not persistent in the female liver, the male hepatic expression increased again at 35
days. Sexual dimorphic DsRed2 expression did not change in gonadectomized male and female Tg-rats. However, female hepatic DsRed2 was induced 72
h after the hypophysectomy. Hepatocytes isolated from the female Tg-rats also revealed DsRed2 induction by 96
h in culture. These results suggest that the pituitary hormone suppresses the female hepatic DsRed2 expression causing the sexual dimorphism of DsRed2 expression. |
| doi_str_mv | 10.1016/j.bbrc.2009.02.119 |
| format | article |
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in vivo fluorescent imaging analysis. The DsRed2 expression decreased gradually in both sexes until 28
days after birth. While DsRed2 expression was not persistent in the female liver, the male hepatic expression increased again at 35
days. Sexual dimorphic DsRed2 expression did not change in gonadectomized male and female Tg-rats. However, female hepatic DsRed2 was induced 72
h after the hypophysectomy. Hepatocytes isolated from the female Tg-rats also revealed DsRed2 induction by 96
h in culture. These results suggest that the pituitary hormone suppresses the female hepatic DsRed2 expression causing the sexual dimorphism of DsRed2 expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2009.02.119</identifier><identifier>PMID: 19250923</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Female ; Hepatocytes ; Hepatocytes - cytology ; Hepatocytes - metabolism ; Hepatocytes - physiology ; Liver - cytology ; Liver - metabolism ; Liver - physiology ; Luminescent Proteins - biosynthesis ; Luminescent Proteins - genetics ; Male ; Pituitary hormone ; Pituitary Hormones - metabolism ; Rats ; Rats, Transgenic ; Red Fluorescent Protein ; Sex Characteristics ; Sexual dimorphism ; Transgenic rat</subject><ispartof>Biochemical and biophysical research communications, 2009-04, Vol.382 (1), p.46-50</ispartof><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-69117be59fd427c8629923ea627c3a35452af73d34691c2d39a2722dab85b4fb3</citedby><cites>FETCH-LOGICAL-c451t-69117be59fd427c8629923ea627c3a35452af73d34691c2d39a2722dab85b4fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19250923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arao, Yukitomo</creatorcontrib><creatorcontrib>Hakamata, Yoji</creatorcontrib><creatorcontrib>Igarashi, Yuka</creatorcontrib><creatorcontrib>Sato, Yuki</creatorcontrib><creatorcontrib>Kayama, Fujio</creatorcontrib><creatorcontrib>Takahashi, Masafumi</creatorcontrib><creatorcontrib>Kobayashi, Eiji</creatorcontrib><creatorcontrib>Murakami, Takashi</creatorcontrib><title>Characterization of hepatic sexual dimorphism in Alb-DsRed2 transgenic rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We previously created the Alb-DsRed2 transgenic (Tg) rat that specifically expresses the red fluorescent protein, DsRed2, in the liver. Herein, we demonstrate that the DsRed2 expression is sexually dimorphic and exhibits a male-specific pattern. The profiling of sexual dimorphism in DsRed2 expression during pre-pubertal development was investigated using an
in vivo fluorescent imaging analysis. The DsRed2 expression decreased gradually in both sexes until 28
days after birth. While DsRed2 expression was not persistent in the female liver, the male hepatic expression increased again at 35
days. Sexual dimorphic DsRed2 expression did not change in gonadectomized male and female Tg-rats. However, female hepatic DsRed2 was induced 72
h after the hypophysectomy. Hepatocytes isolated from the female Tg-rats also revealed DsRed2 induction by 96
h in culture. These results suggest that the pituitary hormone suppresses the female hepatic DsRed2 expression causing the sexual dimorphism of DsRed2 expression.</description><subject>Animals</subject><subject>Female</subject><subject>Hepatocytes</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - metabolism</subject><subject>Hepatocytes - physiology</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver - physiology</subject><subject>Luminescent Proteins - biosynthesis</subject><subject>Luminescent Proteins - genetics</subject><subject>Male</subject><subject>Pituitary hormone</subject><subject>Pituitary Hormones - metabolism</subject><subject>Rats</subject><subject>Rats, Transgenic</subject><subject>Red Fluorescent Protein</subject><subject>Sex Characteristics</subject><subject>Sexual dimorphism</subject><subject>Transgenic rat</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkE1rGzEQhkVpqJ2kfyCHsqfedjsz-2VBL8FNmhJDICTQm9BKs7HMfrjSOjT59ZWxobfkpBE87zvDI8QFQoaA1bdN1jTeZAQgM6AMUX4QcwQJKSEUH8UcAKqUJP6eidMQNgCIRSU_iRlKKkFSPhe3y7X22kzs3aue3DgkY5useRtnkwT-u9NdYl0_-u3ahT5xQ3LZNemPcM-WksnrITzxEFGvp3AuTlrdBf58fM_E4_XVw_ImXd39_LW8XKWmKHFKK4lYN1zK1hZUm0VFMp7CuoqfXOdlUZJu69zm8VY0ZHOpqSayulmUTdE2-Zn4eujd-vHPjsOkehcMd50eeNwFVdWIixh-FyQoZBXXRJAOoPFjCJ5btfWu1_5FIai9a7VRe9dq71oBqeg6hr4c23dNz_Z_5Cg3At8PAEcZz469CsbxYNg6z2ZSdnRv9f8DK5CPAw</recordid><startdate>20090424</startdate><enddate>20090424</enddate><creator>Arao, Yukitomo</creator><creator>Hakamata, Yoji</creator><creator>Igarashi, Yuka</creator><creator>Sato, Yuki</creator><creator>Kayama, Fujio</creator><creator>Takahashi, Masafumi</creator><creator>Kobayashi, Eiji</creator><creator>Murakami, Takashi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090424</creationdate><title>Characterization of hepatic sexual dimorphism in Alb-DsRed2 transgenic rats</title><author>Arao, Yukitomo ; 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Herein, we demonstrate that the DsRed2 expression is sexually dimorphic and exhibits a male-specific pattern. The profiling of sexual dimorphism in DsRed2 expression during pre-pubertal development was investigated using an
in vivo fluorescent imaging analysis. The DsRed2 expression decreased gradually in both sexes until 28
days after birth. While DsRed2 expression was not persistent in the female liver, the male hepatic expression increased again at 35
days. Sexual dimorphic DsRed2 expression did not change in gonadectomized male and female Tg-rats. However, female hepatic DsRed2 was induced 72
h after the hypophysectomy. Hepatocytes isolated from the female Tg-rats also revealed DsRed2 induction by 96
h in culture. These results suggest that the pituitary hormone suppresses the female hepatic DsRed2 expression causing the sexual dimorphism of DsRed2 expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19250923</pmid><doi>10.1016/j.bbrc.2009.02.119</doi><tpages>5</tpages></addata></record> |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Female Hepatocytes Hepatocytes - cytology Hepatocytes - metabolism Hepatocytes - physiology Liver - cytology Liver - metabolism Liver - physiology Luminescent Proteins - biosynthesis Luminescent Proteins - genetics Male Pituitary hormone Pituitary Hormones - metabolism Rats Rats, Transgenic Red Fluorescent Protein Sex Characteristics Sexual dimorphism Transgenic rat |
| title | Characterization of hepatic sexual dimorphism in Alb-DsRed2 transgenic rats |
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