Evaluation of mitochondrial respiratory chain activity in muscle healing by low-level laser therapy

Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. In this work, we evaluated mitochondrial respiratory chain...

Full description

Saved in:
Bibliographic Details
Published in:Journal of photochemistry and photobiology. B, Biology Biology, 2009-05, Vol.95 (2), p.89-92
Main Authors: Silveira, Paulo C.L., Silva, Luciano Acordi da, Fraga, Daiane B., Freitas, Tiago P., Streck, Emilio L., Pinho, Ricardo
Format: Article
Language:English
Subjects:
Animals
ATP
Electron Transport - physiology
Electron Transport - radiation effects
Electron Transport Chain Complex Proteins - metabolism
Electron Transport Complex II - radiation effects
Electron Transport Complex IV - radiation effects
LLLT
Low-Level Light Therapy
Male
Mitochondria, Muscle - metabolism
Mitochondria, Muscle - radiation effects
Mitochondrial respiratory chain
Muscle, Skeletal - injuries
Muscular injury
Rats
Rats, Wistar
Succinate Dehydrogenase - metabolism
Succinate Dehydrogenase - radiation effects
Wound Healing - physiology
Wound Healing - radiation effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury. Male Wistar rats were randomly divided into three groups ( n = 6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5 J/cm 2. Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle-trauma. Our results showed that the activities of complex II and succinate dehydrogenase after 5 days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment. These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.
ISSN:1011-1344
1873-2682
DOI:10.1016/j.jphotobiol.2009.01.004