Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica

Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit ( TPK1). TPK1 was regulated...

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Bibliographic Details
Published in:Fungal genetics and biology 2009-05, Vol.46 (5), p.390-399
Main Authors: Cervantes-Chávez, José A., Kronberg, Florencia, Passeron, Susana, Ruiz-Herrera, José
Format: Article
Language:English
Subjects:
Animals
cAMP-dependent protein kinase
cell growth
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits - genetics
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
dimorphism
DNA, Fungal - chemistry
DNA, Fungal - genetics
Fungal dimorphism
Fungal Proteins - genetics
Gene Deletion
Gene Expression Regulation, Fungal
MAP Kinase Kinase Kinases - genetics
Molecular Sequence Data
Mycelium - growth & development
Pka catalytic subunit
PKA pathway
Saccharomyces cerevisiae Proteins
Sequence Analysis, DNA
sexual reproduction
Signal Transduction
Yarrowia - cytology
Yarrowia - growth & development
Yarrowia - physiology
Yarrowia lipolytica
yeast-like growth
Yeasts - growth & development
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Summary:Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit ( TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka activity increasing during yeast-like growth. tpk1 null mutants were viable and without growth defects, but more sensitive to different stress conditions. Δ tpk1 mutants were mating-deficient, and grew constitutively in the mycelial form, whereas Δ ste11 (Mapkkk-less)/Δ tpk1 double mutants grew in the yeast form, indicating that this is the default growth pattern of the fungus. Our data confirm that MAPK and PKA pathways operate in opposition during the dimorphic behavior of Y. lipolytica, but synergic in mating. These data stress the idea that in different fungi both signal transduction systems may operate distinctly or even be antagonistic or synergic in the coordination of cell responses to different stimuli.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2009.02.005