Selection of cultivation medium for production of late stationary phase serine proteinases from Bacillus intermedius

B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the im...

Full description

Saved in:
Bibliographic Details
Published in:Journal of basic microbiology 2004-12, Vol.44 (6), p.415-423
Main Authors: Balaban, Nelly, Gabdrakhmanova, Leila, Sharipova, Margarita, Sokolova, Evgeniya, Malikova, Lilia, Mardanova, Ayslu, Rudenskaya, Galina, Leshchinskaya, Inna
Format: Article
Language:English
Subjects:
Amino Acids - pharmacology
Bacillus - enzymology
Bacillus - growth & development
Bacillus intermedius
Calcium - pharmacology
Caseins - metabolism
Culture Media
Enzyme Activators - pharmacology
Enzyme Induction
Enzyme Inhibitors - pharmacology
Fungal Proteins - metabolism
Gelatin - metabolism
Gene Expression Regulation, Bacterial - drug effects
Magnesium - pharmacology
Serine Endopeptidases - biosynthesis
Subtilisin - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:B. intermedius have been shown previously to secrete two serine proteinases: glutamyl endopeptidase 2 and subtilisin 2 during the late stationary phase, with maximal levels of the enzymes activities recorded at the 40th and 44th hours of growth, respectively. In the current study, we analyzed the impact of various culture medium components on biosynthesis of these proteinases. Yeast extract and gelatin did not stimulate the enzymes biosynthesis. However, on the medium containing 0.1% casein subtilisin 2 production increased to reach 140%. Biosynthesis of both serine proteinases, produced by B. intermedius at the late stationary phase, were found to be inhibited by individual amino acids, and to be insensitive to catabolite repression. In order to maximise enzyme production, the presence of Ca2+ and Mg2+ at concentration of 5 mM was shown to be necessary. Based on the results of this work, the composition of a complex culture media for the effective production of late stationary phase proteinases by B. intermedius was developed. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.200410426