Characterization of Clostridium species utilizing liquid chromatography/mass spectrometry of intact proteins

A method for biomarker candidate discovery and strain level pathogen characterization using liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization is described. This method was applied to two pathogenic Clostridium species: C. difficile and C. perfringens. Seven marker proteins...

Full description

Saved in:
Bibliographic Details
Published in:Journal of microbiological methods 2009-05, Vol.77 (2), p.152-158
Main Authors: Everley, Robert A., Mott, Tiffany M., Toney, Denise M., Croley, Timothy R.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - analysis
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Biomarker discovery
Biomarkers - analysis
Chromatography, Liquid
Clostridium
Clostridium - chemistry
Clostridium perfringens
Diagnosis, Differential
Electrophoresis, Gel, Pulsed-Field
Electrospray ionization
Fundamental and applied biological sciences. Psychology
Liquid chromatography/mass spectrometry
Microbiology
Proteins
Sensitivity and Specificity
Species Specificity
Spectrometry, Mass, Electrospray Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for biomarker candidate discovery and strain level pathogen characterization using liquid chromatography/mass spectrometry (LC/MS) with electrospray ionization is described. This method was applied to two pathogenic Clostridium species: C. difficile and C. perfringens. Seven marker proteins per species (fourteen total) were successfully implemented to speciate unknowns during a blind study and could enhance serological and genetic approaches by serving as new targets for detection. Two sets of C. perfringens isolates that were 100% similar by pulsed-field gel electrophoresis (PFGE) were distinguished using LC/MS, demonstrating the high specificity of this approach. The use of LC/MS is less labor intensive than PFGE, affords greater specificity than real-time PCR, and requires no primers or antibodies.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2009.01.013