Immunogenicity and efficacy of intradermal tattoo immunization with adenoviral vector vaccines

Abstract Since intradermal delivery of DNA vaccines via tattoo device is an efficient strategy to induce antigen-specific immune responses, we evaluated this route of application for adenoviral vector vaccines in mice. Although expression levels were comparable after i.d. injection and i.d. tattoo i...

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Bibliographic Details
Published in:Vaccine 2009-05, Vol.27 (21), p.2768-2774
Main Authors: Potthoff, A, Schwannecke, S, Nabi, G, Hoffmann, D, Grunwald, T, Wildner, O, Brockmeyer, N.H, Überla, K, Tenbusch, M
Format: Article
Language:English
Subjects:
Adenoviral vectors
Adenoviridae - genetics
Adenoviruses
Allergy and Immunology
Animals
Antibody Formation - immunology
Antigens
Applied microbiology
Biological and medical sciences
Cell Line
Deoxyribonucleic acid
DNA
Female
Fundamental and applied biological sciences. Psychology
Genetic Vectors - administration & dosage
Genetic Vectors - genetics
Genetic Vectors - immunology
Immune system
Immunization
Immunization - methods
Immunogenetics
Immunogenicity
Infections
Injection
Injections, Intradermal
Intradermal vaccination
Mice
Microbiology
Models, Immunological
Monkeys & apes
Plasmids
Proteins
Respiratory syncytial virus
Skin
Tattooing
Tattooing - methods
Tattoos
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Viral infections
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
Viral Vaccines - metabolism
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Summary:Abstract Since intradermal delivery of DNA vaccines via tattoo device is an efficient strategy to induce antigen-specific immune responses, we evaluated this route of application for adenoviral vector vaccines in mice. Although expression levels were comparable after i.d. injection and i.d. tattoo immunization of adenoviral vectors, the tattoo application confined antigen expression to the upper layers of the dermis. Both delivery approaches resulted in strong CD8+ T-cell and humoral immune responses to three different antigens and conferred protection against mucosal challenge with respiratory syncytial virus. However, in contrast to results obtained with DNA vaccines, intradermal tattoo immunization did not provide any obvious advantage in comparison to simple intradermal injection of the adenoviral vector vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2009.03.001