High vitamin intake by Wistar rats during pregnancy alters tissue fatty acid concentration in the offspring fed an obesogenic diet

• Published in: Metabolism, clinical and experimental clinical and experimental, 2009-05, Vol.58 (5), p.722-730
• Main Authors: Reza-López, Sandra A, Anderson, G. Harvey, Szeto, Ignatius M.Y, Taha, Ameer Y, Ma, David W.L
• Format: Article
• Language: English
• Subjects: Adipose Tissue - chemistry; Adipose Tissue - metabolism; Animals; Biological and medical sciences; Brain - metabolism; Endocrinology & Metabolism; Fatty Acids - metabolism; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; Liver - metabolism; Longitudinal Studies; Male; Muscle, Skeletal - chemistry; Muscle, Skeletal - metabolism; Obesity - metabolism; Pregnancy; Random Allocation; Rats; Rats, Wistar; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vitamins - administration & dosage
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2009.01.014

Abstract Diet during pregnancy affects the long-term health of the offspring. Vitamins are known to modulate lipid metabolism, which may be reflected in tissue fatty acid (FA) concentrations. The objective of this study was to investigate the effect of high vitamin intake during pregnancy on tissue FA concentration of the offspring. Wistar rats were fed an AIN-93G diet with either the recommended vitamin or 10-fold higher amounts (HV) during pregnancy. Afterward, offspring were weaned onto an obesogenic diet. Liver, quadriceps, adipose, and brain were collected over 48 weeks. Fatty acid concentration of tissue total lipids was analyzed by gas chromatography. At birth, the liver from HV offspring was higher in monounsaturated, stearic, and arachidonic acids. At weaning, the liver from HV offspring was higher in stearic and oleic acids; and in adipose tissue, n-6 and n-3 FAs were lower only in the male HV offspring ( P < .05). At 12 weeks, HV offspring had higher concentrations of total fat, saturates, monounsaturates, and n-6 FA in muscle ( P < .05), but not in other tissues. At 48 weeks, gestational diet did not affect tissue total lipid FA concentrations; but differences remained in specific tissue phospholipids species. Liver phospholipids from HV offspring were lower in monounsaturates and n-6 FA. Brain phosphatidylethanolamine was higher in oleic, n-6 FA, and docosahexaenoic acid in the HV offspring. Phosphatidylinositol was lower in saturates, monounsaturates, arachidonic, and docosahexaenoic acids only in HV female offspring. These observations demonstrate that high vitamin intake during pregnancy has short- and long-term effects on tissue FA concentration in the offspring.