The b1 isoform of protocadherin-gamma (Pcdhγ) interacts with the microtubule-destabilizing protein SCG10

• Published in: FEBS letters 2004-12, Vol.578 (1), p.175-179
• Main Authors: Gayet, Odile, LaBella, Vincenzo, Henderson, Christopher E., Kallenbach, Sacha
• Format: Article
• Language: English
• Subjects: Animals; Brain - cytology; Brain - metabolism; Cadherins - genetics; Cadherins - metabolism; Cercopithecus aethiops; COS Cells; Growth cone; Intracellular Signaling Peptides and Proteins; Mice; Microtubules - metabolism; Nerve Growth Factors - metabolism; Neurons - cytology; Neurons - metabolism; Protein Isoforms - genetics; Protein Isoforms - metabolism; Protocadherin; Protocadherin-gamma; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; SCG10; Signal Transduction - physiology; Two-hybrid; Two-Hybrid System Techniques
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.10.096

Due to their structural characteristics and their diversity, the 22 members of the protocadherin-gamma (Pcdhγ) family have been suggested to contribute to the establishment of specific connections in the nervous system. Here, we focus on a single isoform, Pcdhγ-b1. Its expression is found in different brain regions and in developing spinal cord it is restricted to scattered cells, whereas all cells are labeled using an antibody that recognizes all Pcdhγ isoforms. As a first step to understanding the signaling mechanisms downstream of Pcdhγ, we identify the microtubule-destabilizing protein SCG10 as a cytoplasmic interactor for Pcdhγ-b1 and other isoforms of the Pcdhγ-b subfamily, and show that SCG10 and Pcdhγ-b1 are found together in certain neuronal growth cones.