Highly Sensitive Determination of microRNA Using Target-Primed and Branched Rolling-Circle Amplification

One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2009-04, Vol.48 (18), p.3268-3272
Main Authors: Cheng, Yongqiang, Zhang, Xian, Li, Zhengping, Jiao, Xiaoxia, Wang, Yucong, Zhang, Yali
Format: Article
Language:English
Subjects:
Fluorescence
fluorescence determination
microRNA
MicroRNAs - analysis
MicroRNAs - chemistry
MicroRNAs - genetics
Mutation
Nucleic Acid Amplification Techniques
RNA Ligase (ATP) - chemistry
rolling‐circle amplification
Sensitivity and Specificity
Viral Proteins - chemistry
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Summary:One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200805665