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A Fully Recombinant System for Activator-dependent Archaeal Transcription
The core components of the archaeal transcription apparatus closely resemble those of eukaryotic RNA polymerase II, while the DNA-binding transcriptional regulators are predominantly of bacterial type. Here we report the construction of an entirely recombinant system for positively regulated archaea...
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| Published in: | The Journal of biological chemistry 2004-12, Vol.279 (50), p.51719-51721 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c438t-5007a78b0250798f863b4fb3eb7109a49d759a96e470105f251721b03296a8503 |
| container_end_page | 51721 |
| container_issue | 50 |
| container_start_page | 51719 |
| container_title | The Journal of biological chemistry |
| container_volume | 279 |
| creator | Ouhammouch, Mohamed Werner, Finn Weinzierl, Robert O J Geiduschek, E Peter |
| description | The core components of the archaeal transcription apparatus closely resemble those of eukaryotic RNA polymerase II, while
the DNA-binding transcriptional regulators are predominantly of bacterial type. Here we report the construction of an entirely
recombinant system for positively regulated archaeal transcription. By omitting individual subunits, or sets of subunits,
from the in vitro assembly of the 12-subunit RNA polymerase from the hyperthermophile Methanocaldococcus jannaschii , we describe a functional dissection of this RNA polymerase II-like enzyme, and its interactions with the general transcription
factor TFE, as well as with the transcriptional activator Ptr2. |
| doi_str_mv | 10.1074/jbc.C400446200 |
| format | article |
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recombinant system for positively regulated archaeal transcription. By omitting individual subunits, or sets of subunits,
from the in vitro assembly of the 12-subunit RNA polymerase from the hyperthermophile Methanocaldococcus jannaschii , we describe a functional dissection of this RNA polymerase II-like enzyme, and its interactions with the general transcription
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the DNA-binding transcriptional regulators are predominantly of bacterial type. Here we report the construction of an entirely
recombinant system for positively regulated archaeal transcription. By omitting individual subunits, or sets of subunits,
from the in vitro assembly of the 12-subunit RNA polymerase from the hyperthermophile Methanocaldococcus jannaschii , we describe a functional dissection of this RNA polymerase II-like enzyme, and its interactions with the general transcription
factor TFE, as well as with the transcriptional activator Ptr2.</description><subject>Archaeal Proteins - genetics</subject><subject>Archaeal Proteins - metabolism</subject><subject>Base Sequence</subject><subject>DNA, Archaeal - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Methanocaldococcus jannaschii</subject><subject>Methanococcus - genetics</subject><subject>Methanococcus - metabolism</subject><subject>Models, Molecular</subject><subject>Protein Subunits</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombination, Genetic</subject><subject>RNA Polymerase II - chemistry</subject><subject>RNA Polymerase II - genetics</subject><subject>RNA Polymerase II - metabolism</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMoWqtXj7IH8bZ18rVJjqVYLRQEreAtJGnWpuxHTbZK_70rLfToXOYwz_vCPAjdYBhhEOxhbd1owgAYKwjACRpgkDSnHH-cogEAwbkiXF6gy5TW0A9T-BxdYM4kl7QYoNk4m26rape9etfWNjSm6bK3Xep8nZVtzMauC9-ma2O-9BvfLH1_Hke3Mt5U2SKaJrkYNl1omyt0Vpoq-evDHqL36eNi8pzPX55mk_E8d4zKLucAwghpgXAQSpayoJaVlnorMCjD1FJwZVThmQAMvCQcC4ItUKIKIznQIbrf925i-7X1qdN1SM5XlWl8u026EJjJQpJ_QSyE4pLhHhztQRfblKIv9SaG2sSdxqD_LOvesj5a7gO3h-atrf3yiB-09sDdHliFz9VPiF7b0LqVrzURSnPQ_VNY0V-9Y4IE</recordid><startdate>20041210</startdate><enddate>20041210</enddate><creator>Ouhammouch, Mohamed</creator><creator>Werner, Finn</creator><creator>Weinzierl, Robert O J</creator><creator>Geiduschek, E Peter</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20041210</creationdate><title>A Fully Recombinant System for Activator-dependent Archaeal Transcription</title><author>Ouhammouch, Mohamed ; 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recombinant system for positively regulated archaeal transcription. By omitting individual subunits, or sets of subunits,
from the in vitro assembly of the 12-subunit RNA polymerase from the hyperthermophile Methanocaldococcus jannaschii , we describe a functional dissection of this RNA polymerase II-like enzyme, and its interactions with the general transcription
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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2004-12, Vol.279 (50), p.51719-51721 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67148682 |
| source | Elsevier ScienceDirect Journals |
| subjects | Archaeal Proteins - genetics Archaeal Proteins - metabolism Base Sequence DNA, Archaeal - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Methanocaldococcus jannaschii Methanococcus - genetics Methanococcus - metabolism Models, Molecular Protein Subunits Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombination, Genetic RNA Polymerase II - chemistry RNA Polymerase II - genetics RNA Polymerase II - metabolism Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic |
| title | A Fully Recombinant System for Activator-dependent Archaeal Transcription |
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