A Candidate Pheromone Receptor and Two Odorant Receptors of the Hawkmoth Manduca sexta

In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time P...

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Bibliographic Details
Published in:Chemical senses 2009-05, Vol.34 (4), p.305-316
Main Authors: Patch, Harland M., Velarde, Rodrigo A., Walden, Kimberly K.O., Robertson, Hugh M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Drosophila melanogaster
Female
Fundamental and applied biological sciences. Psychology
In Situ Hybridization
insect
Male
Manduca - genetics
Manduca sexta
Microscopy, Electron, Scanning
olfaction
Olfaction. Taste
Perception
pheromone
Phylogeny
Psychology. Psychoanalysis. Psychiatry
Psychology. Psychophysiology
receptor
Receptors, Odorant - classification
Receptors, Odorant - genetics
Receptors, Pheromone - classification
Receptors, Pheromone - genetics
Reverse Transcriptase Polymerase Chain Reaction
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Summary:In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5′-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta.
ISSN:0379-864X
1464-3553
DOI:10.1093/chemse/bjp002