Purification and characterization of a new reovirus from the Chinese mitten crab, Eriocheir sinensis

A new reovirus was recently isolated from a freshwater crab, the Chinese mitten crab, Eriocheir sinensis, in China. The complete viral particles are 55 nm in diameter, icosahedral, non‐enveloped and have a mean buoyant density of 1.39 g cm−3 in CsCl gradient. The viral genome is composed of 12 piece...

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Bibliographic Details
Published in:Journal of fish diseases 2004-12, Vol.27 (12), p.687-692
Main Authors: Zhang, S, Shi, Z, Zhang, J, Bonami, J-R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aquareovirus
Base Sequence
Brachyura - virology
Cesium
characterization
China
Chinese mitten crab
Chlorides
Digestive System - ultrastructure
Digestive System - virology
DNA, Complementary - genetics
Electrophoresis
Eriocheir sinensis
Freshwater
Genome, Viral
Gills - ultrastructure
Gills - virology
Microscopy, Electron
Molecular Sequence Data
purification
Reoviridae
Reoviridae - genetics
Reoviridae - ultrastructure
Reovirus
RNA Replicase - genetics
Sequence Analysis, DNA
ultrastructure
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Summary:A new reovirus was recently isolated from a freshwater crab, the Chinese mitten crab, Eriocheir sinensis, in China. The complete viral particles are 55 nm in diameter, icosahedral, non‐enveloped and have a mean buoyant density of 1.39 g cm−3 in CsCl gradient. The viral genome is composed of 12 pieces of dsRNA with an electrophoretic pattern of 3/4/2/3. This virus infects connective tissue of the gills, gut and hepatopancreas. Partial cDNA cloning and sequence analysis showed that the RNA‐dependent RNA polymerase is located in the first RNA segment. From its biochemical, ultrastructural and physicochemical properties, this virus is quite different from the genus Aquareovirus (Reoviridae). It may represent a new genus of Reoviridae, different from the other crab reoviruses, P and W2.
ISSN:0140-7775
1365-2761
DOI:10.1111/j.1365-2761.2004.00587.x