Enhanced production of amidase from Rhodococcus erythropolis MTCC 1526 by medium optimisation using a statistical experimental design

In the present work, statistical experimental methodology was used to enhance the production of amidase from Rhodococcus erythropolis MTCC 1526. R. erythropolis MTCC 1526 was selected through screening of seven strains of Rhodococcus species. The Placket-Burman screening experiments suggested that s...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2009-05, Vol.36 (5), p.671-678
Main Authors: Vaidya, Bhalchandra K, Mutalik, Snehal R, Joshi, Renuka M, Nene, Sanjay N, Kulkarni, Bhaskar D
Format: Article
Language:English
Subjects:
Acids
Amidohydrolases - genetics
Amidohydrolases - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbon
Carbon sources
Chemicals
Culture Media - chemistry
Culture Media - metabolism
Design of experiments
Enzymes
Experimental design
Experimental methods
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Inorganic Chemistry
Life Sciences
Meat
Methods
Microbiology
Models, Statistical
Nitrogen
Nitrogen sources
Optimization
Original Paper
Peptones
Research Design
Rhodococcus
Rhodococcus - chemistry
Rhodococcus - enzymology
Rhodococcus - genetics
Rhodococcus erythropolis
Studies
Variables
Yeast
Yeasts
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Summary:In the present work, statistical experimental methodology was used to enhance the production of amidase from Rhodococcus erythropolis MTCC 1526. R. erythropolis MTCC 1526 was selected through screening of seven strains of Rhodococcus species. The Placket-Burman screening experiments suggested that sorbitol as carbon source, yeast extract and meat peptone as nitrogen sources, and acetamide as amidase inducer are the most influential media components. The concentrations of these four media components were optimised using a face-centred design of response surface methodology (RSM). The optimum medium composition for amidase production was found to contain sorbitol (5 g/L), yeast extract (4 g/L), meat peptone (2.5 g/L), and acetamide (12.25 mM). Amidase activities before and after optimisation were 157.85 units/g dry cells and 1,086.57 units/g dry cells, respectively. Thus, use of RSM increased production of amidase from R. erythropolis MTCC 1526 by 6.88-fold.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-009-0536-9