Direct electrochemical detection of glucose in human plasma on capillary electrophoresis microchips

We developed an electrochemical detector on a hybrid chip for the determination of glucose in human plasma. The microchip system described in this paper consists of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels and an electrode plate. The copper microelectrode is...

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Bibliographic Details
Published in:Electrophoresis 2004-11, Vol.25 (21-22), p.3853-3859
Main Authors: Du, Yan, Yan, Jilin, Zhou, Weihong, Yang, Xiuyong, Wang, Erkang
Format: Article
Language:English
Subjects:
Blood Glucose - analysis
Buffers
Diabetes Mellitus - blood
Diabetes Mellitus - diagnosis
Electrochemical detection
Electrochemistry
Electrodes
Electrophoresis
Electrophoresis, Microchip - methods
Electrophoresis, Microchip - standards
Equipment Design
Glucose
Humans
Microchip
Microelectrode
Miniaturization
Sensitivity and Specificity
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Summary:We developed an electrochemical detector on a hybrid chip for the determination of glucose in human plasma. The microchip system described in this paper consists of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels and an electrode plate. The copper microelectrode is fabricated by selective electroless deposition. The fabrication of the decoupler is performed by platinum electrochemical deposition on the metal film formed by electroless deposition. Factors influencing the performance, including detection potential, separation field strength, and buffer concentration, were studied. The electrodes exhibited good stability and durability in the analytical procedures. Under optimized detection conditions, glucose responded linearly from 10 μM to 1 mM. Finally, glucose in human plasma from three healthy individuals and two diabetics was successfully determined, giving a good prospect for a new clinical diagnostic instrument.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200406072