Allelic status of 1p and 19q in oligodendrogliomas and glioblastomas: multiplex ligation-dependent probe amplification versus loss of heterozygosity

Abstract Identification of the 1p/19q allelic status in gliomas, primarily those with a major oligodendroglial component, has become an excellent molecular complement to tumor histology in order to identify those cases sensitive to chemotherapy. In addition to loss of heterozygosity (LOH), fluoresce...

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Published in:Cancer genetics and cytogenetics 2009-04, Vol.190 (2), p.93-96
Main Authors: Franco-Hernández, Carmen, Martínez-Glez, Victor, de Campos, Jose M, Isla, Alberto, Vaquero, Jesús, Gutiérrez, Manuel, Casartelli, Cacilda, Rey, Juan A
Format: Article
Language:English
Subjects:
Alleles
Brain Neoplasms - genetics
Brain Neoplasms - pathology
Chromosomes, Human, Pair 1 - genetics
Chromosomes, Human, Pair 19 - genetics
Glioblastoma - genetics
Glioblastoma - pathology
Hematology, Oncology and Palliative Medicine
Humans
Loss of Heterozygosity - genetics
Medical Education
Nucleic Acid Amplification Techniques - methods
Oligodendroglioma - genetics
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Summary:Abstract Identification of the 1p/19q allelic status in gliomas, primarily those with a major oligodendroglial component, has become an excellent molecular complement to tumor histology in order to identify those cases sensitive to chemotherapy. In addition to loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), or comparative genomic hybridization (CGH), multiplex ligation-dependent probe amplification (MLPA) has been shown to be an alternative methodology to identify deletions of those chromosome arms. We used MLPA to explore the 1p and 19q allelic constitution in a series of 76 gliomas: 41 tumors with a major oligodendroglial component, 34 glioblastomas, and one low-grade astrocytoma. We compared the MLPA findings of the oligodendroglial cases with those previously obtained using LOH in the same samples. Thirty-eight of 41 oligodendrogliomas displayed identical findings by both LOH and MLPA, and losses at either 1p and/or 19q were identified in 12 of 35 (34%) astrocytic tumors. These findings agree with data previously reported comparing MLPA versus FISH or CGH in gliomas and suggest that MLPA can be used in the identification of the 1p/19q allelic deletions on these brain neoplasms
ISSN:0165-4608
1873-4456
DOI:10.1016/j.cancergencyto.2008.09.017