Identification of bacteriophage types and their carriage in Staphylococcus aureus

Conserved genomic sequences distinctive of Staphylococcus aureus phage types 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enable...

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Bibliographic Details
Published in:Archives of virology 2004-09, Vol.149 (9), p.1689-1703
Main Authors: Pantucek, R, Doskar, J, Ruzickova, V, Kasparek, P, Oracova, E, Kvardova, V, Rosypal, S
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
DNA Primers
DNA, Viral - analysis
DNA, Viral - chemistry
DNA, Viral - isolation & purification
Fundamental and applied biological sciences. Psychology
Genome, Viral
Genomes
Genomics
Hybridization
Lysogeny
Microbiology
Miscellaneous
Molecular biology
Molecular Sequence Data
Polymerase Chain Reaction
prophages
Prophages - classification
Prophages - genetics
Prophages - isolation & purification
Proteins
Sequence Analysis, DNA
Serology
Staphylococcus aureus
Staphylococcus aureus - virology
Staphylococcus Phages - classification
Staphylococcus Phages - genetics
Staphylococcus Phages - isolation & purification
Virology
Virulence
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Summary:Conserved genomic sequences distinctive of Staphylococcus aureus phage types 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages, but also to detect prophages in S. aureus genomes. One to four different prophages were unambiguously detected in experimentally lysogenized S. aureus strains, and substantial variation in prophage content was found in 176 S. aureus clinical strains of different provenance. In addition, by using a comparative genomics approach, all the prophages in the S. aureus genomes sequenced to date could be revealed and classified.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-004-0335-6