In vitro cultivation of early schistosomula of nasal and visceral bird schistosomes (Trichobilharzia spp., Schistosomatidae)

Cercariae of bird schistosomes (Trichobilharzia szidati and Trichobilharzia regenti) were mechanically stimulated to transform to schistosomula and kept in different cultivation media supplemented with duck red blood cells and/or homogenized nervous tissue. The development under in vitro conditions...

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Bibliographic Details
Published in:Parasitology research (1987) 2009-06, Vol.104 (6), p.1445-1452
Main Authors: CHANOVA, Marta, BULANTOVA, Jana, MASLO, Petr, HORAK, Petr
Format: Article
Language:English
Subjects:
Animal Structures - parasitology
Animals
Aves
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Bird Diseases - parasitology
Birds
Culture Media - chemistry
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Immunology
Invertebrates
Medical Microbiology
Microbiology
Nasal Cavity - parasitology
Original Paper
Parasitology - methods
Schistosomatidae - growth & development
Schistosomatidae - isolation & purification
Trematode Infections - parasitology
Trematode Infections - veterinary
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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Summary:Cercariae of bird schistosomes (Trichobilharzia szidati and Trichobilharzia regenti) were mechanically stimulated to transform to schistosomula and kept in different cultivation media supplemented with duck red blood cells and/or homogenized nervous tissue. The development under in vitro conditions was compared with that in vivo, using the following characters: emptying of penetration glands, surface changes, food uptake, and growth of early schistosomula. The results show that the cultivation medium routinely used for human schistosomes is also suitable for mass production of early schistosomula of bird schistosomes, including the unique nasal species--T. regenti. The changes observed resemble those present in worms developing in vivo; therefore, the in vitro produced early schistosomula might be used for further studies of host-parasite interactions.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-009-1343-y