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Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer ( Odocoileus virginianus) from Iowa and Minnesota using four serologic tests
The white-tailed deer ( Odocoileus virginianus) is considered one of the most important wildlife reservoirs of Neospora caninum and Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to N. caninum by four serologic tests including the indirect...
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| Published in: | Veterinary parasitology 2009-05, Vol.161 (3), p.330-334 |
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| container_title | Veterinary parasitology |
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| creator | Dubey, J.P. Jenkins, M.C. Kwok, O.C.H. Zink, R.L. Michalski, M.L. Ulrich, V. Gill, J. Carstensen, M. Thulliez, P. |
| description | The white-tailed deer (
Odocoileus virginianus) is considered one of the most important wildlife reservoirs of
Neospora caninum and
Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to
N. caninum by four serologic tests including the indirect fluorescent antibody (IFA) test (cut-off 1:25),
Neospora caninum agglutination test (cut-off 1:25), an enzyme-linked immunoabsorbent assay, and Western blot (WB). Sera were also tested for antibodies to
T. gondii using the modified agglutination test (cut-off 1:25). Of 62 adult deer from Minnesota antibodies to
T. gondii were found in 20 (32.2%),
N. caninum in 44 (71%), with dual infections in 18 deer. Of 170 (73 fawns, 9 yearlings, 88 adults) deer from Iowa,
T. gondii antibodies were present in 91 (53.5%) with 37.0, 55.6 and 67.0% seropositivity in fawns, yearlings, and adults, respectively. Antibodies to
N. caninum were found in 150 of 170 (88.2%) by any of the 3 tests (99 by Western blots, 135 by ELISA, 106 by IFA, and 118 by NAT). Dual infections with
T. gondii and
N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of
N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed. |
| doi_str_mv | 10.1016/j.vetpar.2009.01.002 |
| format | article |
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Odocoileus virginianus) is considered one of the most important wildlife reservoirs of
Neospora caninum and
Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to
N. caninum by four serologic tests including the indirect fluorescent antibody (IFA) test (cut-off 1:25),
Neospora caninum agglutination test (cut-off 1:25), an enzyme-linked immunoabsorbent assay, and Western blot (WB). Sera were also tested for antibodies to
T. gondii using the modified agglutination test (cut-off 1:25). Of 62 adult deer from Minnesota antibodies to
T. gondii were found in 20 (32.2%),
N. caninum in 44 (71%), with dual infections in 18 deer. Of 170 (73 fawns, 9 yearlings, 88 adults) deer from Iowa,
T. gondii antibodies were present in 91 (53.5%) with 37.0, 55.6 and 67.0% seropositivity in fawns, yearlings, and adults, respectively. Antibodies to
N. caninum were found in 150 of 170 (88.2%) by any of the 3 tests (99 by Western blots, 135 by ELISA, 106 by IFA, and 118 by NAT). Dual infections with
T. gondii and
N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of
N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2009.01.002</identifier><identifier>PMID: 19285809</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>agglutination tests ; animal age ; animal pathogens ; Animals ; antibodies ; Coccidiosis - epidemiology ; Coccidiosis - immunology ; Coccidiosis - parasitology ; Coccidiosis - veterinary ; Deer ; disease reservoirs ; disease surveillance ; Enzyme-linked immunosorbent assay ; Female ; fluorescent antibody technique ; geographical variation ; Indirect fluorescent antibody test ; Iowa - epidemiology ; Minnesota - epidemiology ; mixed infection ; Neospora - immunology ; Neospora agglutination test ; Neospora caninum ; neosporosis ; Odocoileus virginianus ; serodiagnosis ; Seroepidemiologic Studies ; Serologic Tests - veterinary ; seroprevalence ; temporal variation ; Toxoplasma - immunology ; Toxoplasma gondii ; toxoplasmosis ; Toxoplasmosis, Animal - epidemiology ; Toxoplasmosis, Animal - immunology ; transplacental transmission ; Western blot ; Western blotting ; White-tailed deer ; wild animals ; wildlife diseases</subject><ispartof>Veterinary parasitology, 2009-05, Vol.161 (3), p.330-334</ispartof><rights>2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-22e0f24d88f6127239cd75e9e285af500ef7142f76198c9e215ae39049854b983</citedby><cites>FETCH-LOGICAL-c384t-22e0f24d88f6127239cd75e9e285af500ef7142f76198c9e215ae39049854b983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19285809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dubey, J.P.</creatorcontrib><creatorcontrib>Jenkins, M.C.</creatorcontrib><creatorcontrib>Kwok, O.C.H.</creatorcontrib><creatorcontrib>Zink, R.L.</creatorcontrib><creatorcontrib>Michalski, M.L.</creatorcontrib><creatorcontrib>Ulrich, V.</creatorcontrib><creatorcontrib>Gill, J.</creatorcontrib><creatorcontrib>Carstensen, M.</creatorcontrib><creatorcontrib>Thulliez, P.</creatorcontrib><title>Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer ( Odocoileus virginianus) from Iowa and Minnesota using four serologic tests</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The white-tailed deer (
Odocoileus virginianus) is considered one of the most important wildlife reservoirs of
Neospora caninum and
Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to
N. caninum by four serologic tests including the indirect fluorescent antibody (IFA) test (cut-off 1:25),
Neospora caninum agglutination test (cut-off 1:25), an enzyme-linked immunoabsorbent assay, and Western blot (WB). Sera were also tested for antibodies to
T. gondii using the modified agglutination test (cut-off 1:25). Of 62 adult deer from Minnesota antibodies to
T. gondii were found in 20 (32.2%),
N. caninum in 44 (71%), with dual infections in 18 deer. Of 170 (73 fawns, 9 yearlings, 88 adults) deer from Iowa,
T. gondii antibodies were present in 91 (53.5%) with 37.0, 55.6 and 67.0% seropositivity in fawns, yearlings, and adults, respectively. Antibodies to
N. caninum were found in 150 of 170 (88.2%) by any of the 3 tests (99 by Western blots, 135 by ELISA, 106 by IFA, and 118 by NAT). Dual infections with
T. gondii and
N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of
N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed.</description><subject>agglutination tests</subject><subject>animal age</subject><subject>animal pathogens</subject><subject>Animals</subject><subject>antibodies</subject><subject>Coccidiosis - epidemiology</subject><subject>Coccidiosis - immunology</subject><subject>Coccidiosis - parasitology</subject><subject>Coccidiosis - veterinary</subject><subject>Deer</subject><subject>disease reservoirs</subject><subject>disease surveillance</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>fluorescent antibody technique</subject><subject>geographical variation</subject><subject>Indirect fluorescent antibody test</subject><subject>Iowa - epidemiology</subject><subject>Minnesota - epidemiology</subject><subject>mixed infection</subject><subject>Neospora - immunology</subject><subject>Neospora agglutination test</subject><subject>Neospora caninum</subject><subject>neosporosis</subject><subject>Odocoileus virginianus</subject><subject>serodiagnosis</subject><subject>Seroepidemiologic Studies</subject><subject>Serologic Tests - veterinary</subject><subject>seroprevalence</subject><subject>temporal variation</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>toxoplasmosis</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Toxoplasmosis, Animal - immunology</subject><subject>transplacental transmission</subject><subject>Western blot</subject><subject>Western blotting</subject><subject>White-tailed deer</subject><subject>wild animals</subject><subject>wildlife diseases</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u1DAURiMEokPhDRB4hWCRcO0k43hTCVX8VCp00XZteezr4FFiBzuZwgPxnnjISOxYWfp07udrn6J4SaGiQLfv99UB50nFigGICmgFwB4VG9rxumRtC4-LDdTQlA1QflY8S2kPAA1s-dPijArWtR2ITfH7FmOYIh7UgF4jCZZ8w5CmEBXRyju_jER5Q-7CzzANKo2K9MEb53I6u10wDhNxnjx8dzOWs3IDGmIQI3lLbkzQIQdLIgcXe-ed8kt6R2wMI7kKD-pv81fnPaYwK7Ik53tiwxJJylsNoXeazJjm9Lx4YtWQ8MXpPC_uP328u_xSXt98vrr8cF3qumvmkjEEyxrTdXZLGWe10Ia3KDC_VtkWAC2nDbN8S0Wnc0xbhbWARnRtsxNdfV68WXunGH4s-WY5uqRxGJTHsCS55bTlgkEGmxXUMaQU0copulHFX5KCPOqRe7nqkUc9EqjMevLYq1P_shvR_Bs6-cjA6xWwKkjVR5fk_S0DWudKzrg4EhcrgfkfDg6jTNod1RkXUc_SBPf_Hf4AqhOvFg</recordid><startdate>20090512</startdate><enddate>20090512</enddate><creator>Dubey, J.P.</creator><creator>Jenkins, M.C.</creator><creator>Kwok, O.C.H.</creator><creator>Zink, R.L.</creator><creator>Michalski, M.L.</creator><creator>Ulrich, V.</creator><creator>Gill, J.</creator><creator>Carstensen, M.</creator><creator>Thulliez, P.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090512</creationdate><title>Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer ( Odocoileus virginianus) from Iowa and Minnesota using four serologic tests</title><author>Dubey, J.P. ; Jenkins, M.C. ; Kwok, O.C.H. ; Zink, R.L. ; Michalski, M.L. ; Ulrich, V. ; Gill, J. ; Carstensen, M. ; Thulliez, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-22e0f24d88f6127239cd75e9e285af500ef7142f76198c9e215ae39049854b983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>agglutination tests</topic><topic>animal age</topic><topic>animal pathogens</topic><topic>Animals</topic><topic>antibodies</topic><topic>Coccidiosis - epidemiology</topic><topic>Coccidiosis - immunology</topic><topic>Coccidiosis - parasitology</topic><topic>Coccidiosis - veterinary</topic><topic>Deer</topic><topic>disease reservoirs</topic><topic>disease surveillance</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>fluorescent antibody technique</topic><topic>geographical variation</topic><topic>Indirect fluorescent antibody test</topic><topic>Iowa - epidemiology</topic><topic>Minnesota - epidemiology</topic><topic>mixed infection</topic><topic>Neospora - immunology</topic><topic>Neospora agglutination test</topic><topic>Neospora caninum</topic><topic>neosporosis</topic><topic>Odocoileus virginianus</topic><topic>serodiagnosis</topic><topic>Seroepidemiologic Studies</topic><topic>Serologic Tests - veterinary</topic><topic>seroprevalence</topic><topic>temporal variation</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>toxoplasmosis</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Toxoplasmosis, Animal - immunology</topic><topic>transplacental transmission</topic><topic>Western blot</topic><topic>Western blotting</topic><topic>White-tailed deer</topic><topic>wild animals</topic><topic>wildlife diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dubey, J.P.</creatorcontrib><creatorcontrib>Jenkins, M.C.</creatorcontrib><creatorcontrib>Kwok, O.C.H.</creatorcontrib><creatorcontrib>Zink, R.L.</creatorcontrib><creatorcontrib>Michalski, M.L.</creatorcontrib><creatorcontrib>Ulrich, V.</creatorcontrib><creatorcontrib>Gill, J.</creatorcontrib><creatorcontrib>Carstensen, M.</creatorcontrib><creatorcontrib>Thulliez, P.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dubey, J.P.</au><au>Jenkins, M.C.</au><au>Kwok, O.C.H.</au><au>Zink, R.L.</au><au>Michalski, M.L.</au><au>Ulrich, V.</au><au>Gill, J.</au><au>Carstensen, M.</au><au>Thulliez, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer ( Odocoileus virginianus) from Iowa and Minnesota using four serologic tests</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2009-05-12</date><risdate>2009</risdate><volume>161</volume><issue>3</issue><spage>330</spage><epage>334</epage><pages>330-334</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The white-tailed deer (
Odocoileus virginianus) is considered one of the most important wildlife reservoirs of
Neospora caninum and
Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to
N. caninum by four serologic tests including the indirect fluorescent antibody (IFA) test (cut-off 1:25),
Neospora caninum agglutination test (cut-off 1:25), an enzyme-linked immunoabsorbent assay, and Western blot (WB). Sera were also tested for antibodies to
T. gondii using the modified agglutination test (cut-off 1:25). Of 62 adult deer from Minnesota antibodies to
T. gondii were found in 20 (32.2%),
N. caninum in 44 (71%), with dual infections in 18 deer. Of 170 (73 fawns, 9 yearlings, 88 adults) deer from Iowa,
T. gondii antibodies were present in 91 (53.5%) with 37.0, 55.6 and 67.0% seropositivity in fawns, yearlings, and adults, respectively. Antibodies to
N. caninum were found in 150 of 170 (88.2%) by any of the 3 tests (99 by Western blots, 135 by ELISA, 106 by IFA, and 118 by NAT). Dual infections with
T. gondii and
N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of
N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19285809</pmid><doi>10.1016/j.vetpar.2009.01.002</doi><tpages>5</tpages></addata></record> |
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| ispartof | Veterinary parasitology, 2009-05, Vol.161 (3), p.330-334 |
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| source | Elsevier |
| subjects | agglutination tests animal age animal pathogens Animals antibodies Coccidiosis - epidemiology Coccidiosis - immunology Coccidiosis - parasitology Coccidiosis - veterinary Deer disease reservoirs disease surveillance Enzyme-linked immunosorbent assay Female fluorescent antibody technique geographical variation Indirect fluorescent antibody test Iowa - epidemiology Minnesota - epidemiology mixed infection Neospora - immunology Neospora agglutination test Neospora caninum neosporosis Odocoileus virginianus serodiagnosis Seroepidemiologic Studies Serologic Tests - veterinary seroprevalence temporal variation Toxoplasma - immunology Toxoplasma gondii toxoplasmosis Toxoplasmosis, Animal - epidemiology Toxoplasmosis, Animal - immunology transplacental transmission Western blot Western blotting White-tailed deer wild animals wildlife diseases |
| title | Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer ( Odocoileus virginianus) from Iowa and Minnesota using four serologic tests |
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