Transcriptome analysis of quorum-sensing regulation and virulence factor expression in Pseudomonas aeruginosa

The opportunistic pathogen Pseudomonas aeruginosa possesses two well-studied quorum-sensing (QS) systems ( las and rhl) that are important in the production of virulence factors, antibiotic sensitivity, and biofilm development. High-density oligonucleotide microarrays were used to further characteri...

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Bibliographic Details
Published in:Vaccine 2004-12, Vol.22, p.S15-S20
Main Authors: Wagner, Victoria E., Gillis, Richard J., Iglewski, Barbara H.
Format: Article
Language:English
Subjects:
4-Butyrolactone - analogs & derivatives
4-Butyrolactone - metabolism
Adaptation, Physiological
Bacterial Proteins - physiology
Biofilms - growth & development
DNA-Binding Proteins - physiology
Drug Resistance, Bacterial - genetics
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Mutation
Oligonucleotide Array Sequence Analysis
Oxygen - metabolism
Plankton - physiology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - pathogenicity
Pseudomonas aeruginosa - physiology
Quorum-sensing
RNA, Messenger - metabolism
Trans-Activators - physiology
Virulence
Virulence - genetics
Virulence Factors - genetics
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Summary:The opportunistic pathogen Pseudomonas aeruginosa possesses two well-studied quorum-sensing (QS) systems ( las and rhl) that are important in the production of virulence factors, antibiotic sensitivity, and biofilm development. High-density oligonucleotide microarrays were used to further characterize the las QS system and to investigate the effect of environment (planktonic or biofilm mode of growth, absence or presence of oxygen) and nutritional conditions on detection of transcripts encoding QS-regulated virulence factors. Transcriptome results indicate that the QS system is far more complex than previously proposed. Interestingly, we found that many QS-regulated genes encoding virulence products were expressed in all conditions investigated.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2004.08.011