Effect of dequalinium on the oxidative stress in Plasmodium berghei-infected erythrocytes

The bisquinoline drug dequalinium (DQ) has demonstrated remarkable activity against some infection diseases, including malaria. Oxidative stress represents a biochemical target for potential antimalarials. In this work, we have tested the ability of this compound to modify the oxidative status in Pl...

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Bibliographic Details
Published in:Parasitology research (1987) 2009-06, Vol.104 (6), p.1491-1496
Main Authors: Rodrigues, Juan R, Gamboa, Neira D
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents, Local - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Catalase - metabolism
Dequalinium - pharmacology
Erythrocytes - parasitology
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Glucosephosphate Dehydrogenase - metabolism
Glutathione - metabolism
Glutathione Peroxidase - metabolism
Glutathione Transferase - metabolism
Immunology
Invertebrates
Lipid Peroxidation - drug effects
Male
Medical Microbiology
Mice
Mice, Inbred BALB C
Microbiology
Original Paper
Oxidative Stress
Phosphogluconate Dehydrogenase - metabolism
Plasmodium berghei - drug effects
Superoxide Dismutase - metabolism
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Summary:The bisquinoline drug dequalinium (DQ) has demonstrated remarkable activity against some infection diseases, including malaria. Oxidative stress represents a biochemical target for potential antimalarials. In this work, we have tested the ability of this compound to modify the oxidative status in Plasmodium berghei-infected erythrocytes. After hemolysis, activities of superoxide dismutase (SOD), catalase (CAT), glutathione cycle, and dehydrogenase enzymes were investigated. The activity of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGLD) in infected cells were diminished by this drug compared to controls (300% and 80% approximately, respectively), while glutathione peroxidase (GPx), glutathione transferase (GST), and glutathione levels were also lowered. As a compensatory response, we could appreciate an increase of SOD activity (20% approximately) in infected cells treated with DQ; however, catalase was not affected by the compound. Lipid peroxidation was also decreased by this drug, protecting the cells from the hemolysis caused by the infection. In conclusion, oxidative stress represents a biochemical event which is modulated by DQ, interfering with the antioxidant regular activities in P. berghei infection.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-009-1355-7