Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors

By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant g...

Full description

Saved in:
Bibliographic Details
Published in:Transgenic research 2004-10, Vol.13 (5), p.451-461
Main Authors: Huang, S, Gilbertson, L.A, Adams, T.H, Malloy, K.P, Reisenbigler, E.K, Birr, D.H, Snyder, M.W, Zhang, Q, Luethy, M.H
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium tumefaciens
Agrobacterium tumefaciens - genetics
binary plasmid vectors
Biological and medical sciences
Biotechnology
corn
Deoxyribonucleic acid
DNA
DNA, Plant - genetics
Fundamental and applied biological sciences. Psychology
Genes, Reporter - genetics
Genetic engineering
genetic markers
Genetic technics
genetic transformation
Methods. Procedures. Technologies
Plants, Genetically Modified - genetics
plasmid vectors
Plasmids - genetics
Recombination, Genetic
transfer DNA
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
Zea mays
Zea mays - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:By introducing additional T-DNA borders into a binary plasmid used in Agrobacterium-mediated plant transformation, previous studies have demonstrated that the marker gene and the gene of interest (GOI) can be carried by independent T-strands, which sometimes integrate in unlinked loci in the plant genome. This allows the recovery of marker-free transgenic plants through genetic segregation in the next generation. In this study, we have found that by repositioning the selectable marker gene in the backbone and leaving only the GOI in the T-DNA region, a regular two-border binary plasmid was able to generate marker-free transgenic maize plants more efficiently than a conventional single binary plasmid with multiple T-DNA borders. These results also provide evidence that both the right and left borders can initiate and terminate T-strands. Such non-canonical initiation and termination of T-strands may be the basis for the elevated frequencies of cotransformation and unlinked insertions.
ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-004-1453-3