Traditional water mite fixatives and their compatibility with later DNA studies

This work compares frozen water, 70% alcohol, Koenike's and Angelier's fluid as preservation media for water mites in terms of their eventual facilitation of DNA extraction and amplification. The time the mites spent in the fixatives ranged between 1 week and 25 years. Two molecular marker...

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Bibliographic Details
Published in:Experimental & applied acarology 2004-01, Vol.34 (1-2), p.59-65
Main Authors: Rey, I, Dorda, B.A, Valdecasas, A.G
Format: Article
Language:English
Subjects:
alcohols
Amplification
Angelier's fluid
Animals
Base Sequence
chemical preservation
chemical treatment
cytochrome-c oxidase
DNA extraction
DNA, Mitochondrial - analysis
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
duration
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - genetics
Expeditions
extraction
Fixatives
Fixatives - standards
genes
genetic markers
Koenike's fluid
Mites
Mites - chemistry
Mites - genetics
Mitochondrial DNA
Molecular Sequence Data
Nucleotide sequence
preservation media
Preservation, Biological - methods
Preservation, Biological - standards
preservatives
Ribosomal DNA
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
sequence analysis
Sequence Analysis, DNA
Spain
water
water mites
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Description
Summary:This work compares frozen water, 70% alcohol, Koenike's and Angelier's fluid as preservation media for water mites in terms of their eventual facilitation of DNA extraction and amplification. The time the mites spent in the fixatives ranged between 1 week and 25 years. Two molecular markers were amplified: 16S ribosomal DNA and CoI mitochondrial DNA. DNA was extractable and could be sequenced from specimens fixed in all the above media, although this generally became more difficult as time progressed. In the light of the known characteristics of these media, the results suggest Angelier's fluid to be the most practical, especially on long expeditions.
ISSN:0168-8162
1572-9702
DOI:10.1023/B:APPA.0000044439.21180.ec