Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea

Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea. In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effac...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2004-11, Vol.16 (6), p.576-578
Main Authors: Ha, S.K, Hong, K, Choi, C.S, Jung, K, Ha, Y.C, Kim, J.H, Kim, S.H, Yoon, B.S, Chae, C.H
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - genetics
Animals
diarrhea
Diarrhea - microbiology
Diarrhea - veterinary
DNA Primers - genetics
enterovirulent Escherichia coli group
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Escherichia coli Proteins - genetics
Escherichia infections
genes
genetic variation
Genotype
piglets
polymerase chain reaction
Polymerase Chain Reaction - veterinary
strain differences
Swine
Swine Diseases - microbiology
weaning
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Summary:Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea. In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870401600614